AJP - Heart Calcium Transients and Cell-Sarcomere
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 281: H1122-H1130, 2001;
0363-6135/01 $5.00
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Monti, L. D.
Right arrow Articles by Samaja, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Monti, L. D.
Right arrow Articles by Samaja, M.
Vol. 281, Issue 3, H1122-H1130, September 2001

Triglycerides impair postischemic recovery in isolated hearts: roles of endothelin-1 and trimetazidine

Lucilla D. Monti1, Sonia Allibardi5, Pier Marco Piatti2, Gianpietro Valsecchi1, Sabrina Costa1, Guido Pozza3, Sergio Chierchia4, and Michele Samaja5

1 Divisione di Medicina, 2 Unita' di Malattie Metaboliche, Divisione di Medicina, 3 Cattedra di Clinica Medica Generale e Terapia Medica, Universita' Vita-Salute, 4 Dipartimento di Cardiologia, Istituto di Ricovero e Cura a Carattere Scientifico, Hospital San Raffaele, 20132 Milan; and 5 Dipartimento di Medicina, Chirurgia ed Odontoiatria-DiMCO, Ospedale San Paolo, University of Milan, Milan I-20090, Italy


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

There is growing evidence that hypertriglyceridemia exacerbates ischemic injury. We tested the hypothesis that triglycerides impair myocardial recovery from low-flow ischemia in an ex vivo model and that such an effect is related to endothelin-1. Hyperglycemic (glucose concentration = 12 mmol/l) and hyperinsulinemic (insulin concentration = 1.2 µmol/l) isolated rat hearts were perfused with Krebs-Henseleit buffer (PO2 = 670 mmHg, pH 7.4, 37°C) added with increasing triglycerides (0, 1,000, 2,000, and 4,000 mg/dl, n = 6-9 rats/group). Hearts were exposed to 60 min of low-flow ischemia (10% of basal coronary flow), followed by 30 min of reperfusion. We found that increasing triglycerides impaired both the diastolic (P < 0.005) and systolic (P < 0.02) recovery. The release of endothelin-1 during reperfusion increased linearly with triglyceride concentration (P = 0.0009). Elevated triglycerides also increased the release of nitrite and nitrate (NOx), the end products of nitric oxide, up to 6 µmol/min. Trimetazidine (1 µmol) further increased NOx release, blunted endothelin-1 release, and protected myocardial function during recovery. We conclude that high triglyceride levels impair myocardial recovery after low-flow ischemia in association with endothelin-1 release. The endothelium-mediated effect of triglycerides on both contractile recovery and endothelin-1 release is prevented by 1 µM trimetazidine.

nitric oxide


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

ACCUMULATING EPIDEMIOLOGICAL EVIDENCE suggests that the situation characterized by elevated plasma triglycerides (TG) is associated with increased cardiovascular risk independent of factors such as hyperglycemia and elevated plasma cholesterol (3). Hypertriglyceridemia is also a critical risk factor for coronary heart disease (CHD) mortality in subjects with impaired glucose tolerance or diabetes (18). Furthermore, hypertriglyceridemia is a common finding in survivors of acute myocardial infarction (27). This epidemiological evidence suggests that TG influence myocardial performance after ischemia-reperfusion independently of atherosclerosis progression.

Although its role in acute ischemia-reperfusion is controversial, endothelin-1 (ET-1) is known to exacerbate injury, likely via activation of ET type A receptors (9). Studies in isolated hearts showed that ET-1 release increases on early reperfusion after ischemia, thereby contributing to injury (7), and that ET-1 is a major factor that depresses cardiac function (6) and causes cell necrosis (8). These findings are consistent with other studies (21, 23, 30) demonstrating a relationship between ET-1 and the pathogenesis of myocardial ischemia. In humans, acute hypertriglyceridemia stimulates ET-1 release in normal subjects (36). In addition, hypertriglyceridemia is related with elevated plasma levels of ET-1 in glucose-intolerant and type II diabetic patients with insulin resistance syndrome (37). However, on an experimental ground, a link among hypertriglyceridemia, ET-1, and the outcome of the ischemia-reperfusion injury is still lacking. The purpose of this study is to provide experimental evidence of that link by testing the hypothesis that TG exacerbate the injury driven by ischemia-reperfusion and that this phenomenon is linked to ET-1.

The isolated crystalloid-perfused heart may be a suitable model to test this issue in three steps: 1) evaluate the direct acute effect of high TG on postischemic recovery, 2) assess the link between the reperfusion injury and ET-1 release, and 3) evaluate the protection afforded by the piperazine drug trimetazidine (TMZ). No attempt is made to investigate the mechanism underlying ET-1 recognition by cardiac myocytes, because it is known to involve ET type A receptors (9, 10, 19, 43). However, by testing the effect of TMZ, a recognized anti-anginal and anti-ischemic agent (13), one may understand the site of action of TG. Indeed, TMZ inhibits the activity of 3-ketoacyl coenzyme A (CoA) thiolase, the key enzyme of fatty acid beta -oxidation, thereby increasing myocardial oxidative glucose metabolism (29), and the inability to utilize glucose for the oxidative metabolism increases cardiovascular risk in the presence of excess fatty acids (34). Citrate release is a useful index of the flux through the beta -oxidation path (50). In addition, because inactivation of nitric oxide (NO) may play a prominent role in cardiovascular disease (15), we measured the release of nitrite and nitrate (NOx), the end products of NO metabolism, during the reperfusion as a probe to assess the viability of the endothelial cells.

To mimic the metabolic situation occurring in type II diabetic patients during the postprandial period, we selected hyperglycemic and hyperinsulinemic conditions. Indeed, hyperinsulinemia increases plasma ET-1 in humans (36) because insulin stimulates ET-1 secretion from human endothelial (17) and vascular smooth muscle cells (2). In this study, hearts perfused in the presence of increasing TG concentration ([TG]), as well as 10-6 M TMZ, are exposed to low-flow ischemia and reperfused. Data will show that the postischemic injury is proportional to [TG] and ET-1 release and that the deleterious effects of elevated TG are prevented by TMZ.


    MATERIALS AND METHODS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Heart perfusion. Male Sprague-Dawley rats (250-280 g body wt) fed ad libitum were anesthetized with heparinized thiopental sodium (10 mg/100 g body wt). Hearts were excised, immersed in isotonic saline solution (20°C) and mounted on the perfusion system as described previously (44). The time required for these operations never exceeded 45 s and was typically in the 15- to 30-s range. Langendorff perfusion started immediately with the media described below. A peristaltic pump (Gilson; Viliers Le Bel, France) delivered the medium at desired flows to the 8-µm-pore-size filter (47-mm diameter, Nucleopore; Pleasanton, CA), the preheater, and the aortic cannula. All the components of the apparatus, including the heart chamber, the oxygenator, and the preheater, were connected to a 1,760-W external water bath (Endocal, Neslab Instruments; Newington, NH) kept at 37.5 ± 0.5°C. A latex balloon in the left ventricle was connected to a pressure transducer (model 52-9966, Harvard Apparatus; Natick, MA) to monitor myocardial performance (see Experimental protocol). An additional transducer connected to the aortic cannula provided the coronary perfusion pressure (CPP). A cannula was inserted into the pulmonary artery to collect the venous return and to monitor venous PO2 by an O2-sensing electrode (model 5300 Oxygen Monitor, Yellow Springs Instruments; Yellow Springs, OH). The investigation conforms to the guidelines in the Guide for the Care and Use of Laboratory Animals (National Institutes of Health, Publication No. 85-23, Revised 1985).

The perfusion media consisted of a Krebs-Henseleit buffer with 2.0 mmol/l free Ca2+, 12 mmol/l glucose, and 20 mU/l human recombinant insulin (Actrapid HM, Novo Nordisk; Rome, Italy) added with variable amounts (22.5-90 ml/l perfusion medium) of Intralipid 20% (Fresenius Kabi; Verona, Italy). The medium composition was not changed during the protocol. Before dilution, Intralipid 20% contained 200 g/l TG, 12 g/l phospholipids, 25 g/l glycerol, and 257-280 mg/l cholesterol. Linoleic acid is the main fatty acid in TG (18 carbons, 2 cis double bonds), with linolenic, oleic, palmitic, and stearic acids accounting for <50% of the total. Vitamin E present in the mixture partly inhibits oxidation of unsaturated double bonds (G. Arcuri and F. Kabi, unpublished communication).

In control hearts (TG0 group, n = 9), no Intralipid was added to the Krebs-Henseleit buffer. In the TG1,000 (n = 9), TG2,000 (n = 7), and TG4,000 (n = 6) groups, Intralipid was added to the medium to yield [TG] ~1,000, 2,000, and 4,000 mg/dl. The TG4,000 + TMZ (n = 5) group was similar to TG4,000 group, but with 10-6 M freshly prepared TMZ (Servier Laboratories; Courbevoie, France). The medium was equilibrated at PO2 = 670 ± 6 mmHg (means ± SE) and PCO2 = 36 ± 1 mmHg in membrane oxygenators (45). The resulting pH was 7.38 ± 0.01 at 37°C.

Myocardial performance was monitored by a LabView system (National Instruments, Austin, TX) running on Macintosh Quadra 700 (Apple; Cupertino, CA). Measurements included the heart rate (HR), the end-diastolic pressure (EDP), the peak systolic pressure (PSP), the maximal rates of pressure development (+dP/dtmax) and relaxation (-dP/dtmax), and the coronary perfusion pressure (CPP). From these parameters, we derived the left ventricular developed pressure (LVDP = PSP - EDP) and LVDP · HR, which represents the myocardial contractile work. The resistance was calculated as (CPP - EDP)/(flow rate)/(ventricle weight) (11). The O2 uptake was calculated from the arteriovenous PO2 difference and flow rate.

Experimental protocol. All hearts were stabilized for 20 min at a flow rate of 15 ml/min for baseline measurements. During this period, the volume of the intraventricular balloon was adjusted to yield an EDP of 10 ± 1 mmHg and was kept constant afterward. Hearts were then subjected to low-flow ischemia for 60 min by reducing the flow to 1.5 ml/min. After ischemia, hearts were reperfused for 30 min with the same flow rate used during baseline. The recovery of postischemic myocardial performance was evaluated at the end of the reperfusion either as an increase of EDP and CPP above baseline values (Delta EDP and Delta CPP, respectively) or as a percentage of HR, LVDP, +dP/dtmax, -dP/dtmax, and LVDP · HR.

Measurements in the coronary effluent. Glucose was measured by a glucose-oxidase analyzer (Yellow Springs Instruments). Insulin was measured in a single assay [within-assay coefficient of variance (CV)-3.0%, between-assay CV-5.0%] with a microparticle enzyme immunoassay (sensitivity = 6 pmol/l, cross-reactivity with proinsulin <2%; IMX, Abbott Laboratories; Abbott Park, IL). Free fatty acid, TG, citrate, and lactate were measured by automated enzymatic spectrofluorimetric methods adapted to COBAS FARA II (within-assay CV-3.0%, between-assay CV-3.0%; Hoffman-La Roche; Basel, Switzerland).

To measure ET-1, the coronary effluent was collected every 10 min for 30 min during the reperfusion, and the samples were extracted on SepPack C18 minicolumn (Amprep, Amersham International; Buckinghamshire, UK). The eluate was evaporated in a Speed Vac (model SC110, Savant Instruments; Farmingdale, NY). Samples were then reconstituted with 250 µl radioimmunoassay buffer and assayed by a radioimmunoassay kit (Endothelin-1,2 High-sensitivity Assay System; Amersham International). The antiserum was a rabbit anti-ET-1 antibody, and the tracer was 125I-labeled ET-1. The assay sensitivity was 1.25 pg/ml, with a typical within- and between-assay CV = 3.0% and 11.9%, respectively. The total release of ET-1 during the reperfusion was calculated by taking the area under the curves representing ET-1 versus time by the trapezoidal rule and by considering, as a basal value, the ET-1 level measured at the end of ischemia.

NOx was measured by enzymatic catalysis coupled with the Griess reaction (49). As for ET-1, total NOx release during reperfusion was calculated by measuring the area under the curves representing NOx versus time by the trapezoidal rule, after taking the NOx level measured at the end of ischemia as the basal value.

Statistics. Data are expressed as means ± SE. To assess the effect of increasing [TG], we performed a two-way factorial analysis of variance test (StatView, Abacus Concepts; Berkeley, CA). To assess the effects of TMZ at constant [TG], we used Student's t-test. Simple regression analysis was performed using the indices of myocardial performance at recovery as the dependent variables and TG or ET-1 levels as the independent variables.


    RESULTS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

The concentration of glucose and insulin in the media were 12.5 ± 0.5 mmol/l and 1.22 ± 0.03 µmol/l, respectively. The level of free fatty acids was <0.2 mmol/l in both the arterial inflow and venous effluent. Because all hearts kept contracting through the ischemia-reperfusion protocol, all data were available for analysis. Table 1 shows myocardial performance during baseline. All parameters (except for CPP) were not altered by the increase of TG. Although increased in the TG1,000 group, resistance was not further altered for fourfold Intralipid increases. There was no effect of TMZ during baseline except for the higher O2 uptake in the TG4,000 + TMZ group, which reflects the slightly improved, albeit nonsignificant, performance in TMZ hearts. Despite some intergroup differences in the O2 uptake-to-LVDP · HR ratio, which helps to address the relative contribution of carbohydrates and lipids to energy production, there is no [TG]-associated trend or significant effects of TMZ.

                              
View this table:
[in this window]
[in a new window]
  		Table 1.   Myocardial performance during baseline

The exposure of hearts to 60-min low-flow ischemia, followed by 30-min reperfusion, impaired myocardial performance. Increasing [TG] further impaired recovery. Delta EDP increased with increasing [TG] (P = 0.005) up to 38.5 ± 10.7 mmHg (Fig. 1A). Delta CPP tended to increase, although nonsignificantly (P = 0.08). The presence of 10-6 M TMZ in the medium blunted (P = 0.05) the increase in EDP; Delta CPP tended to decrease, although nonsignificantly (P = 0.07) (Fig. 1B). Figure 2 shows other parameters of the ventricular function. HR was not affected by TG, but TMZ increased HR at the end of the postischemic recovery (P = 0.03). The recovery of LVDP was progressively impaired by the increase of [TG] to 55 ± 5% of baseline (P = 0.0008). However, 10-6 M TMZ significantly (P = 0.02) protected hearts and increased the recovery of developed pressure to 81 ± 7% baseline. The same trend as that described for LVDP was also observed for +dP/dtmax, -dP/dtmax, and LVDP · HR.


View larger version (16K):
[in this window]
[in a new window]
  		Fig. 1.   Increase of the end-diastolic pressure (A) and of the coronary perfusion pressures (B) over baseline as measured at the end of reperfusion. Left P values indicate the significance of the trend as a function of increasing triglycerides (one-way factorial analysis of variance test applied to the linear regression). Right P values indicate the significance of the effect of 10-6 M trimetazidine (TMZ) compared with the value corresponding to triglyceride concentration ([triglycerides]) = 4,000 mg/dl. Data are means ± SE. NS, not significant.



View larger version (18K):
[in this window]
[in a new window]
  		Fig. 2.   Recovery of heart rate (HR) (A), left ventricular developed pressure (LVDP) (B), maximal rates of pressure development (C) and relaxation (D), and cross-product LVDP · HR (E), expressed as percentage of the baseline value. Data are means ± SE (see Fig. 1).

Figure 3, A and B, shows that increasing [TG] augments ET-1 release during reperfusion from 0.9 ± 0.2 ng/min in the absence of TG to 6.3 ± 1.6 ng/min in the presence of 4,000 mg/dl TG (P = 0.001). The presence of 10-6 M TMZ completely blunted the release of ET-1 (P = 0.008). Figure 3, C and D, shows that increasing [TG] slightly increased the release of NOx. The release of NOx appeared to be blunted when [TG] = 2,000 mg/dl because a further [TG] increase did not augment NOx release. However, in the presence of 10-6 M TMZ, the release of NOx was augmented threefold with respect to the same [TG] in the absence of TMZ. Figure 3E shows that the release of citrate increases linearly with [TG] and is blunted in the presence of 10-6 M TMZ.


View larger version (13K):
[in this window]
[in a new window]
  		Fig. 3.   Releases of endothelin-1 (ET-1) (A), nitrite and nitrate (B), and citrate (C) during reperfusion. Data are means ± SE (see Fig. 1).

Figure 4, A and B, shows the correlation existing between ET-1 release and EDP or the recovery of LVDP · HR measured at the end of the reperfusion. The correlation is statistically significant (P = 0.0002 and P = 0.01, respectively). Similarly, a significant correlation is found when substituting EDP or LVDP · HR with either LVDP (P = 0.002), +dP/dtmax (P = 0.005), or -dP/dtmax (P = 0.004) but not CPP (P = not significant).


View larger version (23K):
[in this window]
[in a new window]
  		Fig. 4.   Correlation between ET-1 release and end-diastolic pressure (A) or the recovery of LVDP · HR (B) at the end of reperfusion. P = 0.0002 and 0.01, respectively.

Figure 5 shows the changes in the O2 uptake-to-LVDP · HR ratio in the various groups. Low-flow ischemia significantly decreased that ratio in all groups with respect to baseline (P < 0.007). On reperfusion, the O2 uptake-to-LVDP · HR ratio recovered to near-normal values, with the exception of the TG4,000 group, for which that ratio increased from 0.27 ± 0.02 to 0.55 ± 0.05 µmol · mmHg-1 · 1,000 (P = 0.003). Venous lactate concentration ([lactate]) at the end of ischemia ranged from 1.6 to 2.3 mmol/l in all groups without differences among the groups.


View larger version (21K):
[in this window]
[in a new window]
  		Fig. 5.   Oxygen uptake-to-LVDP · HR ratio during the experimental protocol in the groups. That ratio is expressed relative to baseline value to emphasize intergroup variations. #P = 0.003, significant difference with respect to baseline (paired Student's t-test). TG0, no triglycerides added; TG1,000, TG2,000, and TG4,000, triglycerides added to medium to concentrations of 1,000, 2,000, and 4,000 mg/dl; TG4,000 + TMZ, TG4,000 + 10-6 M TMZ.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

In this hyperglycemic, hyperinsulinemic model, elevated [TG] in the medium progressively impairs the postischemic recovery of both systolic and diastolic functions. The impairment is associated with increased ET-1 release. The addition of 10-6 M TMZ protects hearts from the deleterious effect of high TG. The protection is associated with blunted ET-1 release and increased NOx release.

Critique of the model. By reducing to a reasonable minimum the number of the involved variables, the isolated crystalloid-perfused heart model appears suitable for studying the effects of TG in the ischemic-reperfused cardiac muscle. The animals were not pretreated; thus the observations relate to acute metabolic effects. Any interference by neurohormonal factors is excluded because isolated hearts are denervated. Perfusion with blood cell-free media excludes the disturbing effect of neutrophil accumulation and thrombin-induced platelet aggregation. Temperature is strictly controlled (±0.5°C). A constant balloon volume rules out differences in loading conditions. The duration and severity of ischemia are the same in all groups. The changes in vascular resistance are monitored as Delta CPP because the flows were the same in all the groups. Although the selected experimental conditions with relatively short ischemia-reperfusion times do not allow for appreciable no reflow, the slight, nonsignificant increase of vascular resistance shown in Fig. 1 indicate that this phenomenon might have occurred on a longer time basis. The changes in diastolic contracture are reflected by Delta EDP because the balloon volume is fixed at the start of the experiment and kept constant afterward.

Hearts were perfused with media containing glucose and lipids as oxidizable substrates. The experiments reported here were not designed to address the question of their relative contribution to energy production, but the changes in the O2 uptake-to-LVDP · HR ratio reflect different substrate contribution to the rate of ATP synthesis. The fall of that ratio at the end of low-flow ischemia is a consequence of increased substrate-level phosphorylation, as it is evident from the increased venous [lactate]. It is well known that ATP generated from glycolysis represents a substantial portion of total energy production under low-flow ischemia (5, 16); this feature was also verified in the same experimental model as that used in this study (45). However, the O2 uptake-to-LVDP · HR ratio returned to near-normal values at the end of reperfusion except for the TG4,000 group. Thus these hearts shifted from carbohydrate to lipid metabolism, a well-known O2 waste effect (32), to a greater extent than those in the other groups. Interestingly, in the TG4,000 + TMZ group, the ratio was normal, supporting the role of TMZ in shifting heart metabolism toward the use of glucose rather than lipids (29).

TG-associated injury. The deleterious effect of plasma free fatty acids on myocardial postischemic injury in vivo is well known (34). However, the level of free fatty acids in the perfusion media employed in this study was always <0.2 mmol/l. Because of the relatively high flow, free fatty acids were undetectable in the venous effluent. However, it is likely that TG were in part hydrolyzed in the vascular compartment, with release of fatty acids into the cytoplasm. Measurement of the citrate release rate provided evidence of this mechanism because this rate reflects the mitochondrial efflux of citrate and is an index of the concentration of substrates feeding acetyl-CoA and oxaloacetate for the citrate synthase reaction (50). The essentially linear relationship between the citrate release rate and [TG] supports the view that TG are partly hydrolyzed into fatty acids, and fatty acids are uptaken into the myocytes. Intracellular fatty acids are known to depress myocardial recovery from ischemia (28), possibly through increased beta -oxidation and decreased glycolysis and/or glucose oxidation. Measurement of citrate release during reperfusion rules out the potentially masking effects of intracellular citrate concentration peaks that might have occurred during ischemia (24). The blunted citrate release in TMZ-perfused hearts reinforces the hypothesis that the protection afforded by TMZ is exerted through inhibition of beta -oxidation and stimulation of glycolysis and/or glucose oxidation (29). Glycolysis is important in restoring postischemic Ca2+ homeostasis and myocardial function (26), as well as in maintaining membrane integrity (5). This study, however, shows that high TG may impair the postischemic recovery also through increased release of ET-1. Although this hypothesis requires more mechanistic information on how high TG increases ET-1 formation or expression, the present data demonstrates that in this model progressively increased [TG] results in a dose-dependent increase in ET-1 release and that increased ET-1 is highly related to the ischemia-induced performance dysfunction (Fig. 4).

Intralipid is a pool of different types of triglycerides, with fatty acids with variable chain lengths and numbers of cis double bonds. Thus the mechanism of action of TG in the isolated perfused heart requires further work to be elucidated. Previous work (48) pointed out that Intralipid administration during reperfusion is protective, with linoleic acid and phospholipids having complementary actions. However, this situation is different from that in the present study, with Intralipid administered throughout the ischemia-reperfusion protocol. Regardless of the involved mechanism, it was already demonstrated (42) that under normal conditions, the contribution of phospholipids, cholesterol esters, monoacyl glycerols, and diacyl glycerols to myocardial oxidative metabolism in the presence of TG is <5% of the total. Although this work deals with ischemia-reperfusion and not normal conditions, we believe that the effects observed here are to be attributed mainly to TG.

NO. Decreased NO availability plays a significant role in the reperfusion injury even in the absence of blood components, especially at the level of the diastolic function (35). Indeed, supplementation with sodium nitroprusside during hypoxia improves left ventricle relaxation (14) and NO donors inhibit reoxygenation-induced hypercontracture (46). The present data shows that TG increases NOx release, probably by a mechanism analogous to that described in small rabbit arteries, by which NO-mediated, shear-induced dilatation opposes the vasoconstriction elicited by increased pressure (38). Indeed, ischemia and reperfusion cause injury to the vascular endothelium, expressed as a reduction in NO release (47). However, it appears from the data shown Fig. 3 that the increase in NOx is blunted at [TG] = 2,000 mg/dl, thereby reducing the possible cardioprotective effect of NO, which is restored by 10-6 M TMZ. It was shown (31) that at low doses NO may exert a positive inotropic effect on cardiac function, whereas a relaxation-hastening effect of NO becomes apparent while the dose of NO is increased. Therefore, it remains to be established whether the NOx release found in the presence of TMZ falls within the protective NO dose range. If we assume that the release of NOx was constant over time during the 30 min of reperfusion, then the value of 1,200 µmol/l divided by 30 min yields 40 µmol · l-1 · min-1 release, which is apparently beneficial to protect hearts after 60-min low-flow ischemia.

TMZ. We explored the effect of TMZ at concentrations (10-6 M) that were previously found efficient with regard to ischemic protection (4). This concentration is within the therapeutic range because it compares with the blood levels obtained in ischemic patients receiving oral treatment (40). In this study, 10-6 M TMZ inhibits ET-1 secretion, increases the release of NOx, and reduces the deleterious effect of high TG.

Several hypotheses, not necessarily exclusive, have been proposed to explain the effect of TMZ. First, by inhibiting the activity of 3-ketoacyl CoA thiolase and the flow through the beta -oxidation, TMZ increases oxidative glucose metabolism (29) (see TG-associated injury). Second, by sparing energy during ischemia, TMZ preserves the ATP pool (1). Third, TMZ reduces the intracellular acidosis caused by ischemia (39). Fourth, TMZ enhances mitochondrial function (12). Other studies aimed at assessing the effect of TMZ on Na+-K+-ATPase (25) and on mitochondrial Ca2+ uptake (22) showed that this effect occurs only for TMZ levels much higher than those that protect the myocardium. In the present study, it is difficult to assess whether blunted ET-1 release is a consequence of the TMZ protective effect on myocardium or if TMZ inhibits ET-1 release by protecting the endothelium. However, the observation that TMZ greatly increases NOx release strongly supports the hypothesis that in this model part of the protection is exerted at the level of the endothelial cells. Indeed, immunocytochemical studies aimed at localizing NO synthase and ET-1 in the coronary vascular bed showed that both occur in the endothelial cells (41). Furthermore, pressure-induced tone is regulated by NO and ET-1 but no interaction between the two factors was evident because they involve different kinds of receptors, i.e., alpha 1- and alpha 2-adrenoceptors (33).

Study limitation and clinical implications. Although we designed this study to mimic the reduction of coronary blood flow that might occur in atherosclerotic coronary vessels of hyperglycemic, hyperinsulinemic, and hyperlipidemic type II diabetic patients during the postprandial period, extrapolation of our data to the clinical situation is to be made with care. First, responses may be different in normal hearts and hearts from diabetic or hyperlipidemic rats. Second, although uncommon, the situation of [TG] = 4,000 mg/dl may be found in diabetic hyperlipidemic patients in the postprandial period. This situation is worth studying because the link between hypertriglyceridemia and CHD is best seen in the postprandial period, when patients experience exaggerated lipemia, probably related to the delayed clearance of dietary fats (51). The plasma TG level in the postprandial period is positively correlated (3-4 times) with the fasting TG level (20). Furthermore, normalization of plasma TG is markedly delayed in CHD patients because of the presence of gut-derived plasma lipids.

The use of media with increasing Intralipid contents might in principle alter the vascular tone. However, although resistance increased from TG0 to TG1,000, it remained constant up to TG4,000, suggesting that increased viscosity would not have significantly altered data. Although we cannot rule out the possibility that the presence of TG induces vasodilation, possibly via increased NO production, viscosity may not be a central problem. Indeed, the mean globule size for Intralipid is 340 nm (G. Arcuri and F. Kabi, unpublished communication), thus in the same order of magnitude of the size of chilomicrons (75-1,000 nm). However, the selected way to report data and evaluate statistics, i.e., by considering [TG] as a continuous variable, takes this issue into account.

In this study, we did not investigate whether the presence of ET-1 receptor antagonists in the perfusion medium is able to reverse the deleterious effects of high TG on postischemic myocardial function. This important issue clearly deserves further work.

In conclusion, high TG progressively impairs the myocardial recovery from low-flow ischemia. The impairment is significantly related to the release of ET-1, which appears to mediate the mechanism leading to injury. TG also increases the release of NO but not sufficiently to protect the heart from reperfusion injury. By further increasing NO release, 1 µmol/l TMZ prevents ET-1 release and reverses the harmful myocardial effect of TG. By providing experimental evidence of a link among elevated TG, ET-1, and myocardial ischemia-reperfusion injury, this study supports the epidemiological evidence that suggests that the situation characterized by elevated plasma TG is associated with increased cardiovascular risk independent of factors such as hyperglycemia and elevated plasma cholesterol.


    ACKNOWLEDGEMENTS

This study was supported in part by the Ministero dell' Università e della Ricerca Scientifica e Tecnologica Grant "Molecular mechanisms of the protection of the ischemic heart," in part by Italian Ministry of Health Grant RF99.52 "Invalidant Complications of Diabetes," and in part by a grant from the Istituto di Ricovero e Cura a Carattere Scientifico, Hospital San Raffaele.


    FOOTNOTES

Address for reprint requests and other correspondence: L. D. Monti, Divisione di Medicina, IRCCS, Hospital San Raffaele, Via Olgettina 60, 20132 Milano, Italy (E-mail: lucilla.monti{at}hsr.it).

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 29 December 2000; accepted in final form 8 May 2001.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

1.   Allibardi, S, Chierchia S, Margonato V, Merati G, Neri G, Dell'Antonio G, and Samaja M. Effects of trimetazidine on metabolic and functional recovery of post-ischemic rat hearts. Cardiovasc Drugs Ther 12: 543-549, 1998[ISI][Medline].

2.   Anfossi, G, Cavalot F, Massucco P, Mattiello L, Mularoni E, Hahn A, and Trovati M. Insulin influences immunoreactive endothelin release by human vascular smooth muscle. Metabolism 42: 1081-1083, 1993[ISI][Medline].

3.   Austin, MA, McKnight B, Edwards KL, Bradley CM, McNeely MJ, Psaty BM, Brunzell JD, and Motulsky AG. Cardiovascular disease mortality in familial forms of hypetriglyceridemia: a 20-year prospective study. Circulation 101: 2777-2782, 2000[Abstract/Free Full Text].

4.   Boucher, FR, Hearse DJ, and Opie LH. Effects of trimetazidine on ischemic contracture in isolated perfused rat hearts. J Cardiovasc Pharmacol 24: 45-49, 1994[ISI][Medline].

5.   Bricknell, OL, and Opie LH. Effects of substrates on tissue metabolic changes in the isolated rat heart during underperfusion and on release of lactate dehydrogenase and arrhythmias during reperfusion. Circ Res 43: 102-114, 1978[Abstract/Free Full Text].

6.   Brunner, F. Interaction between nitric oxide and endothelin-1 in ischemia/reperfusion injury of rat heart. J Mol Cell Cardiol 29: 2363-2374, 1997[ISI][Medline].

7.   Brunner, F, du Toit EF, and Opie LH. Endothelin release during ischemia and reperfusion of isolated rat hearts. J Mol Cell Cardiol 24: 1291-1305, 1992[ISI][Medline].

8.   Brunner, F, Leonhard B, Kukovetz WR, and Mayer B. Role of endothelin, nitric oxide and L-arginine release in ischemia/reperfusion injury of rat heart. Cardiovasc Res 1997: 60-66, 1997.

9.   Brunner, F, and Opie LH. Role of endothelin A receptors in ischemic contracture and reperfusion injury. Circulation 97: 391-398, 1998[Abstract/Free Full Text].

10.   Caligiuri, G, Levy B, Pernow J, Thoren P, and Hansson GK. Myocardial infarction mediated by endothelin receptor signaling in hypercholesterolemic mice. Proc Natl Acad Sci USA 96: 6920-6924, 1999[Abstract/Free Full Text].

11.   Cunningham, MJ, Apstein CS, Weinberg EO, Vogel WM, and Lorell BH. Influence of glucose and insulin on the exaggerated diastolic and systolic dysfunction of hypertrophied rat hearts during hypoxia. Circ Res 66: 406-415, 1990[Abstract/Free Full Text].

12.   Demaison, L, Fantini E, Sentex E, Grynberg A, and Athias P. Trimetazidine: in vitro influence on heart mitochondrial function. Am J Cardiol 76: 31B-37B, 1995[Medline].

13.   Detry, JMR, and Leclerq PJ. Trimetazidine European multicenter study versus propranolol in stable angina pectoris: contribution of Holter electrocardiographic ambulatory monitoring. Am J Cardiol 76: 8B-11B, 1995[Medline].

14.   Draper, NJ, and Shah AM. Beneficial effects of a nitric oxide donor on recovery of contractile function following brief hypoxia in isolated rat hearts. J Mol Cell Cardiol 29: 1195-1205, 1997[ISI][Medline].

15.   Drexler, H. Nitric oxide and coronary endothelial dysfunction in humans. Cardiovasc Res 43: 572-579, 1999[Free Full Text].

16.   Eberli, FR, Weinberg EO, Grice WN, Horowitz GL, and Apstein CS. Protective effect of increased glycolytic substrate against systolic and diastolic dysfunction and increased coronary resistance from prolonged global underperfusion and reperfusion in isolated rabbit hearts perfused with erythrocyte suspensions. Circ Res 68: 466-481, 1991[Abstract/Free Full Text].

17.   Ferri, C, Pittoni V, Piccoli A, Laurenti O, Cassone MR, Bellini C, Properzi G, Valesini G, De Mattia G, and Santucci A. Insulin stimulates endothelin-1 secretion from human endothelial cells and modulates its circulating levels in vivo. J Clin Endocrinol Metab 80: 829-835, 1995[Abstract].

18.   Fontbonne, A, Eschwege E, Cambien F, Richard JL, Ducimetiere P, Thibult N, Warnet JM, Claude JR, and Rosselin GE. Hypertriglyceridemia as a risk factor of coronary heart disease mortality in subjects with impaired glucose tolerance or diabetes. Diabetologia 32: 300-304, 1989[ISI][Medline].

19.   Gonon, AT, Wang QD, Shimizu M, and Pernow J. The novel non-peptide selective endothelin A receptor antagonist LU 135,252 protects against myocardial ischemic and reperfusion injury in the pig. Acta Physiol Scand 163: 131-137, 1998[ISI][Medline].

20.   Groot, PHE, Van Stiphout WAHJ, and Krauss XH. Postprandial lipoprotein metabolism in normolipemic men with and without coronary disease. Arterioscler Thromb 11: 653-662, 1991[Abstract/Free Full Text].

21.   Grover, GJ, Dzwonczyk S, and Parham CS. The endothelin-1 receptor antagonist BQ-123 reduces infarct size in a canine model of coronary occlusion and reperfusion. Cardiovasc Res 27: 1613-1618, 1993[Abstract/Free Full Text].

22.   Guarnieri, C, Finelli C, Zini M, and Muscari C. Effects of trimetazidine on the calcium transport and oxidative phosphorylation of isolated rat heart mitochondria. Basic Res Cardiol 92: 90-95, 1997[ISI][Medline].

23.   Hasdai, D, Kornowski R, and Battler A. Endothelin and myocardial ischemia. Cardiovasc Drugs Ther 8: 589-599, 1994[ISI][Medline].

24.   Hassel, B, Ilebbek A, and Tonnessen T. Cardiac accumulation of citrate during brief myocardial ischemia and reperfusion in the pig in vivo. Acta Physiol Scand 164: 53-59, 1998[ISI][Medline].

25.   Hisatome, I, Ishiko R, Tanaka Y, Kosaka H, Hasegawa J, Yoshida A, Kotake H, Mashiba H, and Arita M. Trimetazidine inhibits Na+,K+-ATPase activity, and overdrives hyperpolarization in guinea-pig ventricular muscles. Eur J Pharmacol 195: 381-388, 1991[ISI][Medline].

26.   Jeremy, RW, Koretsune Y, Marban E, and Becker LC. Relation between glycolysis and calcium homeostasis in postischemic myocardium. Circ Res 70: 1180-1190, 1992[Abstract/Free Full Text].

27.   Kameda, K, Matsuzawa Y, Kubo M, Ishikawa K, Maejima I, Yamamura T, Yamamoto A, and Tarui S. Increased frequency of lipoprotein disorders similar to type III hyperlipoproteinemia in survivors of myocardial infarction in Japan. Atherosclerosis 51: 241-249, 1984[ISI][Medline].

28.   Kantor, PF, Dyck JRB, and Lopaschuk GD. Fatty acid oxidation in the reperfused ischemic heart. Am J Med Sci 318: 3-14, 1999[ISI][Medline].

29.   Kantor, PF, Lucien A, Kozak R, and Lopaschuk GD. The antianginal drug trimetazidine shifts cardiac energy metabolism from fatty acid oxidation to glucose oxidation by inhibiting mitochondrial long-chain 3-ketoacyl coenzyme A thiolase. Circ Res 86: 580-588, 2000[Abstract/Free Full Text].

30.   Kyriakides, ZS, Markianos M, Iliodromitis EK, and Kremastinos DT. Vein plasma endothelin-1 and cyclic GMP increase during coronary angioplasty is related to myocardial ischemia. Eur Heart J 16: 894-898, 1995[Abstract/Free Full Text].

31.   Mohan, P, Brutsaert DL, Paulus WJ, and Sys SU. Myocardial contractile response to nitric oxide and cGMP. Circulation 93: 1223-1229, 1996[Abstract/Free Full Text].

32.   Neely, JR, and Morgan HE. Relationship between carbohydrate and lipid metabolism and the energy balance of heart muscle. Annu Rev Physiol 36: 413-459, 1974[ISI].

33.   Nguyen, TD, Vequaud P, and Thorin E. Effects of endothelin receptor antagonists and nitric oxide on myogenic tone and alpha adrenergic-dependent contractions of rabbit resistance arteries. Cardiovasc Res 43: 755-761, 1999[Abstract/Free Full Text].

34.   Oliver, MF, and Opie LH. Effects of glucose and fatty acids on myocardial ischemia and arrhrythmias. Lancet 343: 155-158, 1994[ISI][Medline].

35.   Paulus, WJ, and Shah AM. NO and diastolic function. Cardiovasc Res 43: 595-606, 1999[Free Full Text].

36.   Piatti, PM, Monti LD, Conti M, Baruffaldi L, Galli L, Phan CV, Guazzini B, Pontiroli AE, and Pozza G. Hypertriglyceridemia and hyperinsulinemia are potent inducers of endothelin-1 release in humans. Diabetes 45: 316-321, 1996[Abstract].

37.   Piatti, PM, Monti LD, Galli L, Fragasso G, Valsecchi G, Conti M, Gernone F, and Pontiroli AE. Relationship between endothelin-1 concentration and metabolic alterations typical of the insulin resistance syndrome. Metabolism 49: 748-752, 2000[ISI][Medline].

38.   Pohl, U, Herlan K, Huang A, and Bassenge E. Nitric oxide mediated shear-induced dilation opposes myogenic vasoconstriction in small rabit arteries. Am J Physiol Heart Circ Physiol 261: H2016-H2023, 1991[Abstract/Free Full Text].

39.   Reymond, F, Steyaert G, Carrupt PA, Morin D, Tillement JP, Girault HH, and Testa B. The pH-partition profile of the anti-ischemic drug trimetazidine may explain its reduction of intracellular acidosis. Pharm Res 16: 616-624, 1999[ISI][Medline].

40.   Royer, RJ, Royer Morrot MJ, Bannwarth B, Giffard S, and Harpey C. Evaluation des concentrations à l'état d'équilibre et de la fixation globulaire de la trimétazidine. Vastarel 20 mg et l'ischémie myocardique. Gaz Med France 91: 69-70, 1984.

41.   Rubino, A, Loesch A, and Burnstock G. Nitric oxide and endothelin-1 in coronary and pulmonary circulation. Int Rev Cytol 189: 59-93, 1999[ISI][Medline].

42.   Saddik, M, and Lopaschuk GD. Myocardial triglyceride turnover and contribution to energy substrate utilization in isolated working rat hearts. J Biol Chem 266: 8162-8170, 1991[Abstract/Free Full Text].

43.   Sakai, S, Miyauchi T, and Yamaguchi I. Long-term endothelin receptor antagonist administration improves alterations in expression of various cardiac genes in failing myocardium of rats with heart failure. Circulation 101: 2849-2853, 2000[Abstract/Free Full Text].

44.   Samaja, M, Allibardi S, de Jonge R, and Chierchia S. High-energy phosphates metabolism and recovery in reperfused ischemic hearts. Eur J Clin Invest 28: 983-988, 1998[ISI][Medline].

45.   Samaja, M, Casalini S, Allibardi S, Corno A, and Chierchia S. Regulation of bioenergetics in O2-limited isolated rat hearts. J Appl Physiol 77: 2530-2536, 1994[Abstract/Free Full Text].

46.   Schluter, KD, Weber M, Schraven E, and Piper HM. NO donor SIN-1 protects against reoxygenation-induced cardiomyocyte injury by a dual action. Am J Physiol Heart Circ Physiol 267: H1461-H1466, 1994[Abstract/Free Full Text].

47.   Tsao, PS, and Lefer AM. Time course and mechanism of endothelial dysfunction in isolated ischemic- and hypoxic-perfused rat hearts. Am J Physiol Heart Circ Physiol 259: H1660-H1666, 1990[Abstract/Free Full Text].

48.   Van der Velde, M, De Wolff M, and Wouters PF. Effects of lipids on the functional and metabolic recovery from global myocardial stunning in isolated rabbit hearts. Cardiovasc Res 48: 129-137, 2000[Abstract/Free Full Text].

49.   Verdon, CP, Burton BA, and Prior RL. Sample pretreatment with nitrate reductase and glucose-6-phosphate dehydrogenase quantitatively reduces nitrate while avoiding interference by NADP+ when the Griess reaction is used to assay for nitrate. Anal Biochem 224: 502-508, 1995[ISI][Medline].

50.   Vincent, G, Comte B, Poirier M, and Des Rosiers C. Itrate release by perfused rat hearts: a window on mitochondrial cataplerosis. Am J Physiol Endocrinol Metab 278: E846-E856, 2000[Abstract/Free Full Text].

51.   Zilversmit, DB. Atherosclerosis: a postprandial phenomenon. Circulation 60: 473-485, 1979[Abstract/Free Full Text].

Am J Physiol Heart Circ Physiol 281(3):H1122-H1130
0363-6135/01 $5.00 Copyright © 2001 the American Physiological Society


This article has been cited by other articles:


Home page
 J. Thorac. Cardiovasc. Surg.Home page
S. Tamareille, H. Achour, J. Amirian, P. Felli, R. J. Bick, B. Poindexter, Y. J. Geng, W. H. Barry, and R. W. Smalling
Left ventricular unloading before reperfusion reduces endothelin-1 release and calcium overload in porcine myocardial infarction.
J. Thorac. Cardiovasc. Surg., August 1, 2008; 136(2): 343 - 351.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. D. Monti, E. Setola, G. Fragasso, R. P. Camisasca, P. Lucotti, E. Galluccio, A. Origgi, A. Margonato, and P. Piatti
Metabolic and endothelial effects of trimetazidine on forearm skeletal muscle in patients with type 2 diabetes and ischemic cardiomyopathy
Am J Physiol Endocrinol Metab, January 1, 2006; 290(1): E54 - E59.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. D. Monti, C. Landoni, E. Setola, E. Galluccio, P. Lucotti, E. P. Sandoli, A. Origgi, G. Lucignani, P. Piatti, and F. Fazio
Myocardial insulin resistance associated with chronic hypertriglyceridemia and increased FFA levels in Type 2 diabetic patients
Am J Physiol Heart Circ Physiol, September 1, 2004; 287(3): H1225 - H1231.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
G. Milano, A. F. Corno, S. Lippa, L. K. von Segesser, and M. Samaja
Chronic and Intermittent Hypoxia Induce Different Degrees of Myocardial Tolerance to Hypoxia-Induced Dysfunction
Experimental Biology and Medicine, June 1, 2002; 227(6): 389 - 397.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Monti, L. D.
Right arrow Articles by Samaja, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Monti, L. D.
Right arrow Articles by Samaja, M.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online