| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Division of Cardiology and 2Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of California, San Diego, California
Submitted 31 March 2005 ; accepted in final form 13 July 2005
ANG-(1–7) improves the function of the remodeling heart. Although this peptide is generated directly within the myocardium, the effects of ANG-(1–7) on cardiac fibroblasts that play a critical role in cardiac remodeling are largely unknown. We tested the hypothesis that specific binding of ANG-(1–7) to cardiac fibroblasts regulates cellular functions that are involved in cardiac remodeling. 125I-labeled ANG-(1–7) binding assays identified specific binding sites of ANG-(1–7) on adult rat cardiac fibroblasts (ARCFs) with an affinity of 11.3 nM and a density of 131 fmol/mg protein. At nanomolar concentrations, ANG-(1–7) interacted with specific sites that were distinct from ANG II type 1 and type 2 receptors without increasing cytosolic Ca2+ concentration. At these concentrations, ANG-(1–7) had inhibitory effects on collagen synthesis as assessed by [3H]proline incorporation and decreased mRNA expression of growth factors in ARCFs. These effects of ANG-(1–7) contrasted with effects of ANG II. Pretreatment of ARCFs with ANG-(1–7) inhibited ANG II-induced increases in collagen synthesis and in mRNA expression of growth factors, including endothelin-1 and leukemia inhibitory factor. ANG-(1–7) pretreatment also inhibited the stimulatory effects of conditioned medium from ANG II-treated ARCFs on [3H]leucine incorporation and atrial natriuretic factor mRNA expression, markers of hypertrophy, in cardiomyocytes. Thus ANG-(1–7) interacted with specific receptors on ARCFs to exert potential antifibrotic and antitrophic effects that could reverse ANG II effects. These results suggest that ANG-(1–7) may play an important role in the heart in regulating cardiac remodeling.
angiotensin receptors; cardiac remodeling; renin-angiotensin system
This article has been cited by other articles:
|
|
 |
|
  J. A. Stewart Jr, E. Lazartigues, and P. A. Lucchesi The Angiotensin Converting Enzyme 2/Ang-(1-7) Axis in the Heart: A Role for Mas Communication? Circ. Res., November 21, 2008; 103(11): 1197 - 1199. [Full Text] [PDF]
|
|
|
|
 |
|
 B. Greenberg An ACE in the Hole: Alternative Pathways of the Renin Angiotensin System and Their Potential Role in Cardiac Remodeling J. Am. Coll. Cardiol., August 26, 2008; 52(9): 755 - 757. [Full Text] [PDF]
|
|
|
|
 |
|
 B. Greenberg Molecular Imaging of the Remodeling Heart: The Next Step Forward J. Am. Coll. Cardiol. Img., May 1, 2008; 1(3): 363 - 365. [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Giani, M. M. Gironacci, M. C. Munoz, D. Turyn, and F. P. Dominici Angiotensin-(1-7) has a dual role on growth-promoting signalling pathways in rat heart in vivo by stimulating STAT3 and STAT5a/b phosphorylation and inhibiting angiotensin II-stimulated ERK1/2 and Rho kinase activity Exp Physiol, May 1, 2008; 93(5): 570 - 578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Burchill, E. Velkoska, R. G. Dean, R. A. Lew, A. I. Smith, V. Levidiotis, and L. M. Burrell Acute kidney injury in the rat causes cardiac remodelling and increases angiotensin-converting enzyme 2 expression Exp Physiol, May 1, 2008; 93(5): 622 - 630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Filho, A. J. Ferreira, S. H. S. Santos, S. R. S. Neves, E. R. Silva Camargos, L. K. Becker, H. A. Belchior, M. F. Dias-Peixoto, S. V. B. Pinheiro, and R. A. S. Santos Selective increase of angiotensin(1-7) and its receptor in hearts of spontaneously hypertensive rats subjected to physical training Exp Physiol, May 1, 2008; 93(5): 589 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Pan, C.-H. Wen, and C.-S. Lin Interplay of angiotensin II and angiotensin(1-7) in the regulation of matrix metalloproteinases of human cardiocytes Exp Physiol, May 1, 2008; 93(5): 599 - 612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. S. Santos, A. J. Ferreira, and A. C. Simoes e Silva Recent advances in the angiotensin-converting enzyme 2-angiotensin(1-7)-Mas axis Exp Physiol, May 1, 2008; 93(5): 519 - 527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Garabelli, J. G. Modrall, J. M. Penninger, C. M. Ferrario, and M. C. Chappell Distinct roles for angiotensin-converting enzyme 2 and carboxypeptidase A in the processing of angiotensins within the murine heart Exp Physiol, May 1, 2008; 93(5): 613 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Xu, A. C. Costa-Goncalves, M. Todiras, L. A. Rabelo, W. O. Sampaio, M. M. Moura, S. Sousa Santos, F. C. Luft, M. Bader, V. Gross, et al. Endothelial Dysfunction and Elevated Blood Pressure in Mas Gene-Deleted Mice Hypertension, February 1, 2008; 51(2): 574 - 580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. da Costa Goncalves, R. Leite, R. A. Fraga-Silva, S. V. Pinheiro, A. B. Reis, F. M. Reis, R. M. Touyz, R. C. Webb, N. Alenina, M. Bader, et al. Evidence that the vasodilator angiotensin-(1 7)-Mas axis plays an important role in erectile function Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2588 - H2596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Chappell Emerging Evidence for a Functional Angiotensin-Converting Enzyme 2-Angiotensin-(1-7)-Mas Receptor Axis: More Than Regulation of Blood Pressure? Hypertension, October 1, 2007; 50(4): 596 - 599. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Menon, D. R. Soto-Pantoja, M. F. Callahan, J. M. Cline, C. M. Ferrario, E. A. Tallant, and P. E. Gallagher Angiotensin-(1-7) Inhibits Growth of Human Lung Adenocarcinoma Xenografts in Nude Mice through a Reduction in Cyclooxygenase-2 Cancer Res., March 15, 2007; 67(6): 2809 - 2815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. F. Benter, M. H. M. Yousif, C. Cojocel, M. Al-Maghrebi, and D. I. Diz Angiotensin-(1-7) prevents diabetes-induced cardiovascular dysfunction Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H666 - H672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Grobe, A. P. Mecca, H. Mao, and M. J. Katovich Chronic angiotensin-(1-7) prevents cardiac fibrosis in DOCA-salt model of hypertension Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2417 - H2423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A.S. Santos, C. H. Castro, E. Gava, S. V.B. Pinheiro, A. P. Almeida, R. D. de Paula, J. S. Cruz, A. S. Ramos, K. T. Rosa, M. C. Irigoyen, et al. Impairment of In Vitro and In Vivo Heart Function in Angiotensin-(1-7) Receptor Mas Knockout Mice Hypertension, May 1, 2006; 47(5): 996 - 1002. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
