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1 channels in murine arterioles:
differential cellular expression and regulation of diameter
School of Biomedical Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom
The primary objectives of
this study were to reveal cell-specific expression patterns and
functions of voltage-gated K+ channel (KV
1)
subunits in precapillary arterioles of the murine cerebral circulation.
KV1 were detected using peptide-specific antibodies in
immunofluorescence and Western blotting assays. KV1.2 was
localized almost exclusively to endothelial cells, whereas KV1.5 was discretely localized to the nerves and nerve
terminals that innervate the arterioles. KV1.5 also
localized specifically to arteriolar nerves in human pial membrane.
KV1.5 was notable for its absence from smooth muscle cells.
KV1.3, KV1.4, and KV1.6 were
localized to endothelial and smooth muscle cells, although KV1.4 had a low expression level. KV1.1 was not
expressed. Therefore, we show that different cell types of pial
arterioles have distinct physiological expression profiles of
KV1, conferring the possibility of differential
modulation by extracellular and second messengers. Furthermore, we show
recombinant agitoxin-2 and margatoxin are potent vasoconstrictors,
suggesting that KV1 subunits have a major function in
determining arteriolar resistance to blood flow.
K+ channel; endothelium; smooth muscle; vascular nerves; cerebral circulation; mouse
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