AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 281: H223-H231, 2001;
0363-6135/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heaps, C. L.
Right arrow Articles by Parker, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heaps, C. L.
Right arrow Articles by Parker, J. L.
Vol. 281, Issue 1, H223-H231, July 2001

Sarcoplasmic reticulum Ca2+ uptake is impaired in coronary smooth muscle distal to coronary occlusion

Cristine L. Heaps1,2, Michael Sturek2,4, Elmer M. Price1,3, M. Harold Laughlin1,2,3, and Janet L. Parker1,2,5

1 Dalton Cardiovascular Research Center and Departments of 2 Physiology, 3 Veterinary Biomedical Sciences, and 4 Internal Medicine, University of Missouri, Columbia, Missouri 65211; and 5 Department of Medical Physiology, Texas A&M University, College Station, Texas 77843

After chronic occlusion, collateral-dependent coronary arteries exhibit alterations in both vasomotor reactivity and associated myoplasmic free Ca2+ levels that are prevented by chronic exercise training. We tested the hypotheses that coronary occlusion diminishes Ca2+ uptake by the sarcoplasmic reticulum (SR) and that exercise training would prevent impaired SR Ca2+ uptake. Ameroid constrictors were surgically placed around the proximal left circumflex (LCx) artery of female swine 8 wk before initiating 16-wk sedentary (pen confined) or exercise-training (treadmill run) protocols. Twenty-four weeks after Ameroid placement, smooth muscles cells were enzymatically dissociated from both the LCx and nonoccluded left anterior descending (LAD) arteries of sedentary and exercise-trained pigs, and myoplasmic free Ca2+ was studied using fura 2 microfluorometry. After the SR Ca2+ store was partially depleted with caffeine (5 mM), KCl-induced membrane depolarization produced a significant decrease in the time to half-maximal (t1/2) myoplasmic free Ca2+ accumulation in LCx versus LAD cells of sedentary pigs. Furthermore, inhibition of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA; 10 µM cyclopiazonic acid) significantly reduced t1/2 in cells isolated from the LAD but not from the LCx. Exercise training did not prevent the differences in t1/2 myoplasmic free Ca2+ accumulation observed between LCx and LAD cells. Occlusion or exercise training did not alter SERCA protein levels. These results support our hypothesis of impaired SR Ca2+ uptake in coronary smooth muscle cells isolated distal to chronic occlusion. Impaired SR Ca2+ uptake was independent of SERCA protein levels and was not prevented by exercise training.

vascular smooth muscle; calcium; SERCA; exercise training; porcine


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
N. Thengchaisri, R. Shipley, Y. Ren, J. Parker, and L. Kuo
Exercise Training Restores Coronary Arteriolar Dilation to NOS Activation Distal to Coronary Artery Occlusion: Role of Hydrogen Peroxide
Arterioscler. Thromb. Vasc. Biol., April 1, 2007; 27(4): 791 - 798.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. L. Heaps, M. L. Mattox, K. A. Kelly, C. J. Meininger, and J. L. Parker
Exercise training increases basal tone in arterioles distal to chronic coronary occlusion
Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H1128 - H1135.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Terai, Y. Hiramoto, M. Masaki, S. Sugiyama, T. Kuroda, M. Hori, I. Kawase, and H. Hirota
AMP-Activated Protein Kinase Protects Cardiomyocytes against Hypoxic Injury through Attenuation of Endoplasmic Reticulum Stress
Mol. Cell. Biol., November 1, 2005; 25(21): 9554 - 9575.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. L. Heaps, J. L. Parker, M. Sturek, and D. K. Bowles
Altered calcium sensitivity contributes to enhanced contractility of collateral-dependent coronary arteries
J Appl Physiol, July 1, 2004; 97(1): 310 - 316.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. K. Bowles, C. L. Heaps, J. R. Turk, K. K. Maddali, and E. M. Price
Hypercholesterolemia inhibits L-type calcium current in coronary macro-, not microcirculation
J Appl Physiol, June 1, 2004; 96(6): 2240 - 2248.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online