| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Cardiology, Hospital General Vall d'Hebron, Barcelona 08035, Spain; and 2 Department of Pharmacology, University College of Cork, Ireland
During myocardial ischemia, severe ATP
depletion induces rigor contracture followed by intracellular
Ca2+ concentration ([Ca2+]i) rise
and progressive impairment of gap junction (GJ)-mediated electrical
coupling. Our objective was to investigate whether chemical coupling
through GJ allows propagation of rigor in cardiomyocytes and
whether it persists after rigor development. In end-to-end connected
adult rat cardiomyocytes submitted to simulated ischemia the
interval between rigor onset was 3.7 ± 0.7 s, and subsequent [Ca2+]i rise was virtually identical in both
cells, whereas in nonconnected cell pairs the interval was 71 ± 12 s and the rate of [Ca2+]i rise was
highly variable. The GJ blocker 18
-glycyrrhetinic acid increased the
interval between rigor onset and the differences in
[Ca2+]i between connected cells. Transfer of
Lucifer yellow demonstrated GJ permeability 10 min after rigor onset in
connected cell pairs, and 30 min after rigor onset in isolated rat
hearts submitted to nonflow ischemia but was abolished after
2 h of ischemia. GJ-mediated communication allows
propagation of rigor in ischemic myocytes and persists after
rigor development despite acidosis and increased [Ca2+]i.
propagation; rigor contracture; calcium
This article has been cited by other articles:
|
|
 |
|
  M. Ruiz-Meana, A. Rodriguez-Sinovas, A. Cabestrero, K. Boengler, G. Heusch, and D. Garcia-Dorado Mitochondrial connexin43 as a new player in the pathophysiology of myocardial ischaemia-reperfusion injury Cardiovasc Res, January 15, 2008; 77(2): 325 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Miura, T. Yano, K. Naitoh, M. Nishihara, T. Miki, M. Tanno, and K. Shimamoto {delta}-Opioid receptor activation before ischemia reduces gap junction permeability in ischemic myocardium by PKC-{varepsilon}-mediated phosphorylation of connexin 43 Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1425 - H1431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rodriguez-Sinovas, D. Garcia-Dorado, M. Ruiz-Meana, and J. Soler-Soler Protective effect of gap junction uncouplers given during hypoxia against reoxygenation injury in isolated rat hearts Am J Physiol Heart Circ Physiol, February 1, 2006; 290(2): H648 - H656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rodriguez-Sinovas, D. Garcia-Dorado, M. Ruiz-Meana, and J. Soler-Soler Enhanced effect of gap junction uncouplers on macroscopic electrical properties of reperfused myocardium J. Physiol., August 15, 2004; 559(1): 245 - 257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Schulz and G. Heusch Connexin 43 and ischemic preconditioning Cardiovasc Res, May 1, 2004; 62(2): 335 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miura, Y. Ohnuma, A. Kuno, M. Tanno, Y. Ichikawa, Y. Nakamura, T. Yano, T. Miki, J. Sakamoto, and K. Shimamoto Protective role of gap junctions in preconditioning against myocardial infarction Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H214 - H221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Eloff, E. Gilat, X. Wan, and D. S. Rosenbaum Pharmacological Modulation of Cardiac Gap Junctions to Enhance Cardiac Conduction: Evidence Supporting a Novel Target for Antiarrhythmic Therapy Circulation, December 23, 2003; 108(25): 3157 - 3163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Padilla, D. Garcia-Dorado, A. Rodriguez-Sinovas, M. Ruiz-Meana, J. Inserte, and J. Soler-Soler Protection afforded by ischemic preconditioning is not mediated by effects on cell-to-cell electrical coupling during myocardial ischemia-reperfusion Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H1909 - H1916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-M. Lee and T.-F. Chou Troglitazone administration limits infarct size by reduced phosphorylation of canine myocardial connexin43 proteins Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1650 - H1659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kanno, A. Kovacs, K. A. Yamada, and J. E. Saffitz Connexin43 as a determinant of myocardial infarct size following coronary occlusion in mice J. Am. Coll. Cardiol., February 19, 2003; 41(4): 681 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Schwanke, I. Konietzka, A. Duschin, X. Li, R. Schulz, and G. Heusch No ischemic preconditioning in heterozygous connexin43-deficient mice Am J Physiol Heart Circ Physiol, October 1, 2002; 283 (4): H1740 - H1742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Garcia-Dorado, M. Ruiz-Meana, F. Padilla, A. Rodriguez-Sinovas, and M. Mirabet Gap junction-mediated intercellular communication in ischemic preconditioning Cardiovasc Res, August 15, 2002; 55(3): 456 - 465. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
