AJP - Heart Myographs and Tissue organ baths
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 280: H2126-H2135, 2001;
0363-6135/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (35)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Manevich, Y.
Right arrow Articles by Fisher, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Manevich, Y.
Right arrow Articles by Fisher, A. B.
Vol. 280, Issue 5, H2126-H2135, May 2001

Oxidative burst and NO generation as initial response to ischemia in flow-adapted endothelial cells

Yefim Manevich, Abu Al-Mehdi, Vladimir Muzykantov, and Aron B. Fisher

Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104-6068

Shear stress modulates endothelial physiology, yet the effect(s) of flow cessation is poorly understood. The initial metabolic responses of flow-adapted bovine pulmonary artery endothelial cells to the abrupt cessation of flow (simulated ischemia) was evaluated using a perfusion chamber designed for continuous spectroscopy. Plasma membrane potential, production of reactive O2 species (ROS), and intracellular Ca2+ and nitric oxide (NO) levels were measured with fluorescent probes. Within 15 s after flow cessation, flow-adapted cells, but not cells cultured under static conditions, showed plasma membrane depolarization and an oxidative burst with generation of ROS that was inhibited by diphenyleneiodonium. EGTA-inhibitable elevation of intracellular Ca2+ and NO were observed at ~30 and 60 s after flow cessation, respectively. NO generation was decreased in the presence of inhibitors of NO synthase and calmodulin. Thus flow-adapted endothelial cells sense the altered hemodynamics associated with flow cessation and respond by plasma membrane depolarization, activation of NADPH oxidase, Ca2+ influx, and activation of Ca2+/calmodulin-dependent NO synthase. This signaling response is unrelated to cellular anoxia.

reduced nicotinamide adenine dinucleotide phosphate oxidase; plasma membrane potential; shear stress; intracellular calcium; O2 consumption


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Milovanova, S. Chatterjee, Y. Manevich, I. Kotelnikova, K. DeBolt, M. Madesh, J. S. Moore, and A. B. Fisher
Lung endothelial cell proliferation with decreased shear stress is mediated by reactive oxygen species
Am J Physiol Cell Physiol, January 1, 2006; 290(1): C66 - C76.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Q. Zhang, I. Matsuzaki, S. Chatterjee, and A. B. Fisher
Activation of endothelial NADPH oxidase during normoxic lung ischemia is KATP channel dependent
Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L954 - L961.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
I. Matsuzaki, S. Chatterjee, K. DeBolt, Y. Manevich, Q. Zhang, and A. B. Fisher
Membrane depolarization and NADPH oxidase activation in aortic endothelium during ischemia reflect altered mechanotransduction
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H336 - H343.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J.-M. Li and A. M Shah
Endothelial cell superoxide generation: regulation and relevance for cardiovascular pathophysiology
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2004; 287(5): R1014 - R1030.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
E. A. Jaimes, E. G. DeMaster, R.-X. Tian, and L. Raij
Stable Compounds of Cigarette Smoke Induce Endothelial Superoxide Anion Production via NADPH Oxidase Activation
Arterioscler. Thromb. Vasc. Biol., June 1, 2004; 24(6): 1031 - 1036.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Teng, S. Kurata, I. Katoh, G. S. Georgieva, T. Nosaka, C. Mitaka, and T. Imai
Cytokine mRNA expression in unilateral ischemic-reperfused rat lung with salt solution supplemented with low-endotoxin or standard bovine serum albumin
Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L137 - L142.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Chatterjee, A.-B. Al-Mehdi, I. Levitan, T. Stevens, and A. B. Fisher
Shear stress increases expression of a KATP channel in rat and bovine pulmonary vascular endothelial cells
Am J Physiol Cell Physiol, October 1, 2003; 285(4): C959 - C967.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. P. Nase, J. Tuttle, and H. G. Bohlen
Reduced perivascular PO2 increases nitric oxide release from endothelial cells
Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H507 - H515.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Z. Wei, A. B. Al-Mehdi, and A. B. Fisher
Signaling pathway for nitric oxide generation with simulated ischemia in flow-adapted endothelial cells
Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H2226 - H2232.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Song, A. B. Al-Mehdi, and A. B. Fisher
An immediate endothelial cell signaling response to lung ischemia
Am J Physiol Lung Cell Mol Physiol, October 1, 2001; 281(4): L993 - L1000.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. B. Fisher, S. Chien, A. I. Barakat, and R. M. Nerem
Endothelial cellular response to altered shear stress
Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L529 - L533.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online