| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Biomedical Engineering Laboratory, Swiss Federal Institute of Technology, 1015 Lausanne; and 2 Department of Zoology and Animal Biology, University of Geneva, 1211 Geneva, Switzerland
The goal of the
present study was to analyze the intercellular calcium communication
between smooth muscle cells (SMCs) and endothelial cells (ECs) by
simultaneously monitoring artery diameter and intracellular calcium
concentration in a rat mesenteric arterial segment in vitro under
physiological pressure (50 mmHg) and flow (50 µl/min) in a specially
developed system. Intracellular calcium was expressed as the fura 2 ratio. The diameter was measured using a digital image acquisition
system. Stimulation of SMCs with the 
1-agonist
phenylephrine (PE) caused not only an increase in the free
intracellular calcium concentration of the SMCs as expected but also in
the ECs, suggesting a calcium flux from the SMCs to the ECs. The gap
junction uncoupler palmitoleic acid greatly reduced this increase in
calcium in the ECs on stimulation of the SMCs with PE. This indicates
that the signaling pathway passes through the gap junctions. Similarly,
although vasomotion originates in the SMCs, calcium oscillates in both
SMCs and ECs during vasomotion, suggesting again a calcium flux from
the SMCs to the ECs.
smooth muscle cells; endothelial cells; calcium imaging; gap junctions; rat mesenteric artery
This article has been cited by other articles:
|
|
 |
|
  M. Koenigsberger, R. Sauser, D. Seppey, J.-L. Beny, and J.-J. Meister Calcium Dynamics and Vasomotion in Arteries Subject to Isometric, Isobaric, and Isotonic Conditions Biophys. J., September 15, 2008; 95(6): 2728 - 2738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Haddock, T. H. Grayson, T. D. Brackenbury, K. R. Meaney, C. B. Neylon, S. L. Sandow, and C. E. Hill Endothelial coordination of cerebral vasomotion via myoendothelial gap junctions containing connexins 37 and 40 Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2047 - H2056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. V. Matchkov, A. Rahman, L. M. Bakker, T. M. Griffith, H. Nilsson, and C. Aalkjaer Analysis of effects of connexin-mimetic peptides in rat mesenteric small arteries Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H357 - H367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. I. Gokina and T. Goecks Upregulation of endothelial cell Ca2+ signaling contributes to pregnancy-enhanced vasodilation of rat uteroplacental arteries Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H2124 - H2135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E Haddock and C. E Hill Rhythmicity in arterial smooth muscle J. Physiol., August 1, 2005; 566(3): 645 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. H. Mauban and W. G. Wier Essential role of EDHF in the initiation and maintenance of adrenergic vasomotion in rat mesenteric arteries Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H608 - H616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-A. Haefliger, P. Nicod, and P. Meda Contribution of connexins to the function of the vascular wall Cardiovasc Res, May 1, 2004; 62(2): 345 - 356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lamboley, A. Schuster, J.-L. Beny, and J.-J. Meister Recruitment of smooth muscle cells and arterial vasomotion Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H562 - H569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Dora, J. Xia, and B. R. Duling Endothelial cell signaling during conducted vasomotor responses Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H119 - H126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Morio, E. P. Carter, M. Oka, and I. F. McMurtry EDHF-mediated vasodilation involves different mechanisms in normotensive and hypertensive rat lungs Am J Physiol Heart Circ Physiol, May 1, 2003; 284(5): H1762 - H1770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. G. Geary, J. N. Buchholz, and W. J. Pearce Maturation depresses mouse cerebrovascular tone through endothelium-dependent mechanisms Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2003; 284(3): R734 - R741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Ungvari, A. Csiszar, and A. Koller Increases in endothelial Ca2+ activate KCa channels and elicit EDHF-type arteriolar dilation via gap junctions Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1760 - H1767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tare, H. A. Coleman, and H. C. Parkington Glycyrrhetinic derivatives inhibit hyperpolarization in endothelial cells of guinea pig and rat arteries Am J Physiol Heart Circ Physiol, January 1, 2002; 282(1): H335 - H341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Marrelli Mechanisms of endothelial P2Y1- and P2Y2-mediated vasodilatation involve differential [Ca2+]i responses Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1759 - H1766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Budel, A. Schuster, N. Stergiopoulos, J.-J. Meister, and J.-L. Beny Role of smooth muscle cells on endothelial cell cytosolic free calcium in porcine coronary arteries Am J Physiol Heart Circ Physiol, September 1, 2001; 281(3): H1156 - H1162. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
