| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 University of Texas Health Science Center, San Antonio, Texas 78284-7872; and 2 The University of Chicago, Chicago, Illinois 60637-1479
The application of left ventricular pressure-volume
analysis to transgenic mice to characterize the cardiac phenotype has been problematic due to the small size of the mouse heart and the rapid
heartbeat. Conductance technology has been miniaturized for the mouse
and can solve this problem. However, there has been no validation of
this technique. Accordingly, we performed echocardiography followed by
simultaneous ultrasonic crystals, flow probe, and conductance studies
in 18 CD-1 mice. Raw conductance volumes were corrected for an
inhomogenous electrical field (
) and parallel conductance
(Gpi) yielding a stroke volume of 14.1 ± 3.7 µl/beat, end-diastolic volume of 20.8 ± 6.5 µl, and
end-systolic volume of 9.0 ± 5.8 µl. The mean conductance
volumes were no different from those derived by flow probe and
echocardiography but did differ from ultrasonic crystals.
Gpi was determined to be 14.9 ± 8.7 µl.
However, hypertonic saline altered dimension and pressure in the mouse
left ventricle. Although Gpi can be determined
by the hypertonic saline method, saline altered hemodynamics,
questioning its validity in the mouse. Although mean measures of
absolute volume may be similar among different techniques, individual
values did not correlate.
conductance catheter; pressure-volume analysis; ultrasonic crystals; electrocardiography; transgenic mouse
This article has been cited by other articles:
|
|
 |
|
  R. Bauer, G.A. MacGowan, A. Blain, K. Bushby, and V. Straub Steroid treatment causes deterioration of myocardial function in the {delta}-sarcoglycan-deficient mouse model for dilated cardiomyopathy Cardiovasc Res, September 1, 2008; 79(4): 652 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Shioura, D. L. Geenen, and P. H. Goldspink Assessment of cardiac function with the pressure-volume conductance system following myocardial infarction in mice Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2870 - H2877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Nielsen, S. B. Kristiansen, S. Ringgaard, T. T. Nielsen, A. Flyvbjerg, A. N. Redington, and H. E. Botker Left ventricular volume measurement in mice by conductance catheter: evaluation and optimization of calibration Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H534 - H540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Reyes, M. E. Steinhelper, J. A. Alvarez, D. Escobedo, J. Pearce, J. W. Valvano, B. H. Pollock, C.-L. Wei, A. Kottam, D. Altman, et al. Impact of physiological variables and genetic background on myocardial frequency-resistivity relations in the intact beating murine heart Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1659 - H1669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Jegger, X. Jeanrenaud, M. Nasratullah, P.-G. Chassot, A. Mallik, H. Tevaearai, L. K. von Segesser, P. Segers, and N. Stergiopulos Noninvasive Doppler-derived myocardial performance index in rats with myocardial infarction: validation and correlation by conductance catheter Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1540 - H1548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Claessens, D. Georgakopoulos, M. Afanasyeva, S. J. Vermeersch, H. D. Millar, N. Stergiopulos, N. Westerhof, P. R. Verdonck, and P. Segers Nonlinear isochrones in murine left ventricular pressure-volume loops: how well does the time-varying elastance concept hold? Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1474 - H1483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Lindsey, G. P. Escobar, L. W. Dobrucki, D. K. Goshorn, S. Bouges, J. T. Mingoia, D. M. McClister Jr., H. Su, J. Gannon, C. MacGillivray, et al. Matrix metalloproteinase-9 gene deletion facilitates angiogenesis after myocardial infarction Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H232 - H239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. MacGowan, J. Rager, S. G. Shroff, and M. A. Mathier In vivo {alpha}-adrenergic responses and troponin I phosphorylation: anesthesia interactions J Appl Physiol, April 1, 2005; 98(4): 1163 - 1170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Pacher, J. G. Mabley, L. Liaudet, O. V. Evgenov, A. Marton, G. Hasko, M. Kollai, and C. Szabo Left ventricular pressure-volume relationship in a rat model of advanced aging-associated heart failure Am J Physiol Heart Circ Physiol, November 1, 2004; 287(5): H2132 - H2137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Berry, T. J. Pirolli, V. Jayasankar, J. Burdick, K. J. Morine, T. J. Gardner, and Y. J. Woo Apelin Has In Vivo Inotropic Effects on Normal and Failing Hearts Circulation, September 14, 2004; 110(11_suppl_1): II-187 - II-193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Jayasankar, L. T. Bish, T. J. Pirolli, M. F. Berry, J. Burdick, and Y. J. Woo Local myocardial overexpression of growth hormone attenuates postinfarction remodeling and preserves cardiac function Ann. Thorac. Surg., June 1, 2004; 77(6): 2122 - 2129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. H. Cingolani, X.-P. Yang, Y.-H. Liu, M. Villanueva, N.-E. Rhaleb, and O. A. Carretero Reduction of Cardiac Fibrosis Decreases Systolic Performance Without Affecting Diastolic Function in Hypertensive Rats Hypertension, May 1, 2004; 43(5): 1067 - 1073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ishizaka, R. E. Sievers, B.-Q. Zhu, M. C. Rodrigo, S. Joho, E. Foster, P. C. Simpson, and W. Grossman New technique for measurement of left ventricular pressure in conscious mice Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H1208 - H1215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Woldbaek, T. A. Stromme, J. B. Sande, G. Christensen, T. Tonnessen, and A. Ilebekk Evaluation of a new fiber-optic pressure recording system for cardiovascular measurements in mice Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H2233 - H2239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Collins, C. E. Korcarz, and R. M. Lang Use of echocardiography for the phenotypic assessment of genetically altered mice Physiol Genomics, May 13, 2003; 13(3): 227 - 239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. H. Cingolani, X.-P. Yang, M. A. Cavasin, and O. A. Carretero Increased Systolic Performance With Diastolic Dysfunction in Adult Spontaneously Hypertensive Rats Hypertension, February 1, 2003; 41(2): 249 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nemoto, G. DeFreitas, D. L. Mann, and B. A. Carabello Effects of changes in left ventricular contractility on indexes of contractility in mice Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2504 - H2510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Nishio, S. Sasayama, and A. Matsumori Left ventricular pressure-volume relationship in a murine model of congestive heart failure due to acute viral myocarditis J. Am. Coll. Cardiol., October 16, 2002; 40(8): 1506 - 1514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Weiss, C. Korcarz, O. Chassande, K. Cua, P. M. Sadow, E. Koo, J. Samarut, and R. Lang Thyroid hormone and cardiac function in mice deficient in thyroid hormone receptor-alpha or -beta : an echocardiograph study Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E428 - E435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. A. Janssen and J. F. M. Smits Autonomic control of blood pressure in mice: basic physiology and effects of genetic modification Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1545 - R1564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Lorenz A practical guide to evaluating cardiovascular, renal, and pulmonary function in mice Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1565 - R1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Janssen, J. Debets, P. Leenders, and J. Smits Chronic measurement of cardiac output in conscious mice Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2002; 282(3): R928 - R935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Eucker, J. Lisauskas, M. R. Courtois, and S. J. Kovacs Analysis of left ventricular hemodynamics in physiological hyperspace J Appl Physiol, January 1, 2002; 92(1): 323 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. E.J.M. Creemers, J. P.M. Cleutjens, J. F.M. Smits, and M. J.A.P. Daemen Matrix Metalloproteinase Inhibition After Myocardial Infarction: A New Approach to Prevent Heart Failure? Circ. Res., August 3, 2001; 89(3): 201 - 210. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
