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Am J Physiol Heart Circ Physiol 278: H444-H451, 2000;
0363-6135/00 $5.00
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Vol. 278, Issue 2, H444-H451, February 2000

Measurements of calcium transients in ventricular cells during discontinuous action potential conduction

Mary B. Wagner, Yang-Gan Wang, Rajiv Kumar, David A. Golod, William N. Goolsby, and Ronald W. Joyner

Todd Franklin Cardiac Research Laboratory, The Children's Heart Center, Department of Pediatrics, Emory University, Atlanta, Georgia 30322

The L-type calcium current (ICa) is important in sustaining propagation during discontinuous conduction. In addition, ICa is altered during discontinuous conduction, which may result in changes in the intracellular calcium transient. To study this, we have combined the ability to monitor intracellular calcium concentration ([Ca2+]i) in an isolated cardiac cell using confocal scanning laser fluorescence microscopy with our "coupling clamp" technique, which allows action potential propagation from the real cell to a real-time simulation of a model cell. Coupling a real cell to a model cell with a value of coupling conductance (GC = 8 nS) just above the critical value for action potential propagation results in both an increased amplitude and an increased rate of rise of the calcium transient. Similar but smaller changes in the calcium transient are caused by increasing GC to 20 nS. The increase of [Ca2+]i by discontinuous conduction is less than the increase of ICa, which may indicate that much of [Ca2+]i is the result of calcium released from the sarcoplasmic reticulum rather than the integration of ICa.

confocal laser scanning microscopy; fluo 3; coupling clamp


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