| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
mediates shear stress-dependent
activation of JNK in endothelial cells
1 Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294; and 2 Max Planck Research Unit Molecular Cell Biology, University of Jena, 07747 Jena, Germany
Shear stress differentially activates
extracellular signal-regulated kinase (ERK) and c-Jun
NH2-terminal kinase (JNK) by
mechanisms involving G
i2 and
G
/
proteins, respectively, in bovine aortic endothelial cells
(BAEC). The early events in this signaling mechanism by which G
proteins regulate ERK and JNK in response to shear stress have not been
defined. Here we show that BAEC endogenously express a G
protein-dependent form of phosphatidylinositol 3-kinase, PI3K, and
its activity is stimulated by shear stress. PI3K activity was
measured in vitro using BAEC that were transiently transfected with an
epitope-tagged PI3K (vsv-PI3K). Exposure of BAEC to shear stress
rapidly and transiently stimulated the activity of vsv-PI3K (maximum
by 15 s, with a return to basal after 1-min exposure to 5 dyn/cm2 shear stress). Activity of
vsv-PI3K was stimulated by shear stress intensities as low as 0.5 dyn/cm2. Treatment of BAEC with an
inhibitor of PI3K, wortmannin, inhibited shear-dependent activation of
JNK but had no effect on that of ERK. Furthermore, expression of a
kinase-inactive mutant
(PI3KK799R) in BAEC inhibited
the shear-dependent activation of JNK but not ERK. Taken together,
these results suggest that PI3K selectively regulates the
shear-sensitive JNK pathway. This differential and novel signaling
pathway may be responsible for coordinating various mechanosensitive
events in endothelial cells.
mechanotransduction; extracellular signal-regulated kinase; G proteins; atherosclerosis
This article has been cited by other articles:
|
|
 |
|
  G. Estilo, W. Liu, N. Pastor-Soler, P. Mitchell, M. D. Carattino, T. R. Kleyman, and L. M. Satlin Effect of aldosterone on BK channel expression in mammalian cortical collecting duct Am J Physiol Renal Physiol, September 1, 2008; 295(3): F780 - F788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Miyazaki, K. Honda, and H. Ohata Requirement of Ca2+ influx- and phosphatidylinositol 3-kinase-mediated m-calpain activity for shear stress-induced endothelial cell polarity Am J Physiol Cell Physiol, October 1, 2007; 293(4): C1216 - C1225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, J. Chang, Y.-C. Li, Y.-S. Li, J. Y.-J. Shyy, and S. Chien Shear stress and VEGF activate IKK via the Flk-1/Cbl/Akt signaling pathway Am J Physiol Heart Circ Physiol, February 1, 2004; 286(2): H685 - H692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. C. Boo and H. Jo Flow-dependent regulation of endothelial nitric oxide synthase: role of protein kinases Am J Physiol Cell Physiol, September 1, 2003; 285(3): C499 - C508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Fleming and R. Busse Molecular mechanisms involved in the regulation of the endothelial nitric oxide synthase Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2003; 284(1): R1 - R12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Oldenhof, O. P. Shynlova, M. Liu, B. L. Langille, and S. J. Lye Mitogen-activated protein kinases mediate stretch-induced c-fos mRNA expression in myometrial smooth muscle cells Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1530 - C1539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. C. Boo, J. Hwang, M. Sykes, B. J. Michell, B. E. Kemp, H. Lum, and H. Jo Shear stress stimulates phosphorylation of eNOS at Ser635 by a protein kinase A-dependent mechanism Am J Physiol Heart Circ Physiol, November 1, 2002; 283(5): H1819 - H1828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-M. Go, Y. C. Boo, H. Park, M. C. Maland, R. Patel, K. A. Pritchard Jr., Y. Fujio, K. Walsh, V. Darley-Usmar, and H. Jo Protein kinase B/Akt activates c-Jun NH2-terminal kinase by increasing NO production in response to shear stress J Appl Physiol, October 1, 2001; 91(4): 1574 - 1581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Kim, B. Gallis, and M. A. Corson TNF-{alpha} inhibits flow and insulin signaling leading to NO production in aortic endothelial cells Am J Physiol Cell Physiol, May 1, 2001; 280(5): C1057 - C1065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kito, E. L. Chen, X. Wang, M. Ikeda, N. Azuma, N. Nakajima, V. Gahtan, and B. E. Sumpio Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain J Appl Physiol, December 1, 2000; 89(6): 2391 - 2400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Yamboliev, K. M. Wiesmann, C. A. Singer, J. C. Hedges, and W. T. Gerthoffer Phosphatidylinositol 3-kinases regulate ERK and p38 MAP kinases in canine colonic smooth muscle Am J Physiol Cell Physiol, August 1, 2000; 279(2): C352 - C360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Park, Y.-M. Go, R. Darji, J.-W. Choi, M. P. Lisanti, M. C. Maland, and H. Jo Caveolin-1 regulates shear stress-dependent activation of extracellular signal-regulated kinase Am J Physiol Heart Circ Physiol, April 1, 2000; 278(4): H1285 - H1293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Go, R. P. Patel, M. C. Maland, H. Park, J. S. Beckman, V. M. Darley-Usmar, and H. Jo Evidence for peroxynitrite as a signaling molecule in flow-dependent activation of c-Jun NH2-terminal kinase Am J Physiol Heart Circ Physiol, October 1, 1999; 277(4): H1647 - H1653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Macrez, C. Mironneau, V. Carricaburu, J.-F. Quignard, A. Babich, C. Czupalla, B. Nurnberg, and J. Mironneau Phosphoinositide 3-Kinase Isoforms Selectively Couple Receptors to Vascular L-Type Ca2+ Channels Circ. Res., October 12, 2001; 89(8): 692 - 699. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
