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current activated by cell swelling in rabbit portal vein vascular
smooth muscle cells
Department of Pharmacology and Clinical Pharmacology, St. George's Cardiovascular Research Group, St. George's Hospital Medical School, London SW17 0RE, United Kingdom
In rabbit portal vein smooth
muscle cells, application of a hypotonic external solution caused cell
swelling and evoked an outwardly rectifying
Cl
current. The
hypotonicity-activated current was markedly reduced by the
anti-estrogen tamoxifen (10 µM) and was inhibited by DIDS in a
voltage-dependent manner [the concentration required to inhibit the current by 50% (IC50) at
50 and +100 mV was 21 and 5 µM DIDS, respectively].
Indanyloxyacetic acid 94 (IAA-94) and niflumic acid also inhibited the
hypotonicity-activated current, with 50% inhibition produced at
concentrations of ~200 and 100 µM, respectively. In isotonic
conditions, application of tamoxifen and DIDS to cells decreased the
holding current due to the inhibition of a resting conductance that was
outwardly rectifying and reversed at the Cl equilibrium potential.
These data show that rabbit portal vein myocytes have a resting
Cl conductance that is
enhanced by cell swelling; its possible physiological role is discussed.
resting conductance; chloride current pharmacology; tamoxifen
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