AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 274: H366-H374, 1998;
0363-6135/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kameyama, T.
Right arrow Articles by Lewinter, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kameyama, T.
Right arrow Articles by Lewinter, M. M.
Vol. 274, Issue 1, H366-H374, January 1998

Mechanoenergetic studies in isolated mouse hearts

Tomoki Kameyama, Zengyi Chen, Stephen P. Bell, Judit Fabian, and Martin M. Lewinter

Cardiology Unit, College of Medicine, The University of Vermont, Burlington, Vermont 05401

We tested the feasibility of an isolated, balloon-in-ventricle, isovolumically contracting, crystalloid-perfused mouse heart preparation (n = 10) for studies of cardiac mechanoenergetics using the end-systolic pressure-volume relation (ESPVR) and myocardial oxygen consumption (VO2)-pressure-volume area (PVA) framework employed in larger species. The intraventricular balloon method was shown to be accurate for measurement of left ventricular volume, especially at relatively higher volumes. The ESPVR demonstrated contractility-dependent curvilinearity. Average slope of the ESPVR was 1,299 ± 369 (SD) mmHg · g · ml-1, with a volume intercept of 0.018 ± 0.006 ml. The VO2-PVA relation was well fitted by a straight line, with average slope and VO2 intercept of 3.57 ± 1.31 × 10-5 ml O2 · mmHg-1 · ml-1 and 0.92 ± 0.21 × 10-3 ml O2 · beat-1 · g-1, respectively. Decreasing perfusate Ca2+ concentration resulted in a decrease in the slope of the ESPVR, a decrease in the VO2 intercept of the VO2-PVA relation, but no significant change in its slope. Hearts from hypothyroid (n = 8) mice demonstrated similar mechanoenergetic changes. We conclude that delineation of the ESPVR and the VO2-PVA relation is feasible in the mouse heart. Our method should allow an assessment of cardiac mechanoenergetics as sophisticated as that previously possible only in larger hearts.

myocardial oxygen consumption; pressure-volume area; ventricular volume


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. D. Belke, B. Gloss, J. M. Hollander, E. A. Swanson, H. Duplain, and W. H. Dillmann
In vivo gene delivery of HSP70i by adenovirus and adeno-associated virus preserves contractile function in mouse heart following ischemia-reperfusion
Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H2905 - H2910.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. E. Claessens, D. Georgakopoulos, M. Afanasyeva, S. J. Vermeersch, H. D. Millar, N. Stergiopulos, N. Westerhof, P. R. Verdonck, and P. Segers
Nonlinear isochrones in murine left ventricular pressure-volume loops: how well does the time-varying elastance concept hold?
Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1474 - H1483.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
O.-J. How, E. Aasum, S. Kunnathu, D. L. Severson, E. S. P. Myhre, and T. S. Larsen
Influence of substrate supply on cardiac efficiency, as measured by pressure-volume analysis in ex vivo mouse hearts
Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2979 - H2985.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. C. Baker, R. Wolk, B.-R. Choi, S. Watkins, P. Plan, A. Shah, and G. Salama
Effects of mechanical uncouplers, diacetyl monoxime, and cytochalasin-D on the electrophysiology of perfused mouse hearts
Am J Physiol Heart Circ Physiol, October 1, 2004; 287(4): H1771 - H1779.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Yamashita, H. Kohzuki, Y. Kitagawa, T. Nakashima, A. Kikuta, and M. Takaki
O2 consumption of mechanically unloaded contractions of mouse left ventricular myocardial slices
Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H54 - H62.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Kitagawa, D. Yamashita, H. Ito, and M. Takaki
Reversible effects of isoproterenol-induced hypertrophy on in situ left ventricular function in rat hearts
Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H277 - H285.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. A. Gustafson and J. H. G. M. Van Beek
Measurement of the activation time of oxidative phosphorylation in isolated mouse hearts
Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H3118 - H3123.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
F. Sam, D. B. Sawyer, D. L.-F. Chang, F. R. Eberli, S. Ngoy, M. Jain, J. Amin, C. S. Apstein, and W. S. Colucci
Progressive left ventricular remodeling and apoptosis late after myocardial infarction in mouse heart
Am J Physiol Heart Circ Physiol, July 1, 2000; 279(1): H422 - H428.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online