| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Heart and Circulatory Physiology, Vol 272, Issue 4 1730-H1740, Copyright © 1997 by American Physiological Society
ARTICLES |
R. R. Magness, C. E. Shaw, T. M. Phernetton, J. Zheng and I. M. Bird
Department of Obstetrics and Gynecology/Perinatal Research Laboratories, University of Wisconsin-Madison, 53715, USA. rmagness@facstaff.wisc.edu
Pregnancy is characterized by elevations in uterine but not omental artery nitric oxide synthase (NOS)-specific activity. We hypothesized that increases in NO production during pregnancy are associated with elevations in protein expression of the constitutive isoform, endothelial cell NOS (ecNOS), in uterine but not systemic arteries. Arterial NOS-specific activity and guanosine 3',5'-cyclic monophosphate (cGMP) production were tested in pregnant sheep in the presence or absence [+5 mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid] of Ca2+. With the use of Western analysis, ecNOS and neuronal NOS (nNOS) constitutive isoform expressions were evaluated in intact and denuded [vascular smooth muscle (VSM)] uterine and systemic (omental and renal) arteries as well as in isolated endothelium-derived proteins from nonpregnant and pregnant sheep. Uterine and omental artery NOS activity and cGMP production were inhibited 75-85% by Ca2+ removal. ecNOS was localized only in uterine and systemic artery endothelium (not VSM) by immunohistochemistry and Western analysis; nNOS was not detected. Compared with nonpregnant ewes, pregnancy increased expression of ecNOS in uterine [2.1- to 4.2-fold (P < 0.0001)] and omental [1.3- to 2.2-fold (P = 0.032)] but not renal (P = 0.1367) artery endothelium. Increases in uterine were greater than in omental artery endothelium. Levels of plasma and urinary cGMP were elevated (P < 0.01) proportionally (1.8- to 2.0-fold) in pregnant versus nonpregnant ewes. During pregnancy, expression of uterine artery endothelium-derived (not VSM) ecNOS constitutive isoform is increased, whereas expression in systemic vessels shows little or no change.
This article has been cited by other articles:
|
|
 |
|
  P.-A. Scott, A. Tremblay, M. Brochu, and J. St-Louis Vasorelaxant action of 17 -estradiol in rat uterine arteries: role of nitric oxide synthases and estrogen receptors Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3713 - H3719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Li, C. E. Wood, and M. Keller-Wood Adrenalectomy alters regulation of blood pressure and endothelial nitric oxide synthase in sheep: modulation by estradiol Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R257 - R266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xiao, X. Huang, L. D. Longo, W. J. Pearce, and L. Zhang Regulation of baseline Ca2+ sensitivity in permeabilized uterine arteries: effect of pregnancy Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H413 - H420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Vonnahme, M. E. Wilson, Y. Li, H. L. Rupnow, T. M. Phernetton, S. P. Ford, and R. R. Magness Circulating levels of nitric oxide and vascular endothelial growth factor throughout ovine pregnancy J. Physiol., May 15, 2005; 565(1): 101 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-X. Yi, R. R. Magness, and I. M. Bird Simultaneous imaging of [Ca2+]i and intracellular NO production in freshly isolated uterine artery endothelial cells: effects of ovarian cycle and pregnancy Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R140 - R148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Gibson, T. M. Phernetton, M. C. Wiltbank, and R. R. Magness Development and Use of an Ovarian Synchronization Model to Study the Effects of Endogenous Estrogen and Nitric Oxide on Uterine Blood Flow During Ovarian Cycles in Sheep Biol Reprod, June 1, 2004; 70(6): 1886 - 1894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kwon, G. Wu, C. J. Meininger, F. W. Bazer, and T. E. Spencer Developmental Changes in Nitric Oxide Synthesis in the Ovine Placenta Biol Reprod, March 1, 2004; 70(3): 679 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Bird, L. Zhang, and R. R. Magness Possible mechanisms underlying pregnancy-induced changes in uterine artery endothelial function Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2003; 284(2): R245 - R258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Joyce, T. M. Phernetton, and R. R. Magness Effect of Uterine Blood Flow Occlusion on Shear Stress-Mediated Nitric Oxide Production and Endothelial Nitric Oxide Synthase Expression During Ovine Pregnancy Biol Reprod, July 1, 2002; 67(1): 320 - 326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xiao, X. Huang, S. Bae, C. A. Ducsay, and L. Zhang Cortisol-mediated potentiation of uterine artery contractility: effect of pregnancy Am J Physiol Heart Circ Physiol, July 1, 2002; 283(1): H238 - H246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Wang, S. Datta, and S. Segal Pregnancy Alters Adrenergic Mechanisms in Uterine Arterioles of Rats Anesth. Analg., May 1, 2002; 94(5): 1304 - 1309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Rupnow, T. M. Phernetton, M. L. Modrick, M. C. Wiltbank, I. M. Bird, and R. R. Magness Endothelial Vasodilator Production by Uterine and Systemic Arteries. VIII. Estrogen and Progesterone Effects on cPLA2, COX-1, and PGIS Protein Expression Biol Reprod, February 1, 2002; 66(2): 468 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Joyce, T. M. Phernetton, C. E. Shaw, M. L. Modrick, and R. R. Magness Endothelial vasodilator production by uterine and systemic arteries. IX. eNOS gradients in cycling and pregnant ewes Am J Physiol Heart Circ Physiol, January 1, 2002; 282(1): H342 - H348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Buhimschi, G. Hall, L. P. Thompson, and C. P. Weiner Pregnancy and estradiol decrease GTPase activity in the guinea pig uterine artery Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H2168 - H2175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yoshida, N. Sagawa, H. Itoh, S. Yura, D. Korita, K. Kakui, N. Hirota, T. Sato, A. Ito, and S. Fujii Nitric oxide increases matrix metalloproteinase-1 production in human uterine cervical fibroblast cells Mol. Hum. Reprod., October 1, 2001; 7(10): 979 - 985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Rosenfeld Mechanisms regulating angiotensin II responsiveness by the uteroplacental circulation Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2001; 281(4): R1025 - R1040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xiao, W. J. Pearce, and L. Zhang Pregnancy enhances endothelium-dependent relaxation of ovine uterine artery: role of NO and intracellular Ca2+ Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H183 - H190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Rosenfeld, D. N. Cornfield, and T. Roy Ca2+-activated K+ channels modulate basal and E2{beta}-induced rises in uterine blood flow in ovine pregnancy Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H422 - H431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Thompson and C. P. Weiner Pregnancy enhances G protein activation and nitric oxide release from uterine arteries Am J Physiol Heart Circ Physiol, May 1, 2001; 280(5): H2069 - H2075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Magness, J. A. Sullivan, Y. Li, T. M. Phernetton, and I. M. Bird Endothelial vasodilator production by uterine and systemic arteries. VI. Ovarian and pregnancy effects on eNOS and NOx Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1692 - H1698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Rupnow, T. M. Phernetton, C. E. Shaw, M. L. Modrick, I. M. Bird, and R. R. Magness Endothelial vasodilator production by uterine and systemic arteries. VII. Estrogen and progesterone effects on eNOS Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1699 - H1705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, K. G. Stewart, and S. T. Davidge Endogenous estrogen mediates vascular reactivity and distensibility in pregnant rat mesenteric arteries Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H956 - H961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xiao, I. M. Bird, R. R. Magness, L. D. Longo, and L. Zhang Upregulation of eNOS in pregnant ovine uterine arteries by chronic hypoxia Am J Physiol Heart Circ Physiol, February 1, 2001; 280(2): H812 - H820. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. M. Miller Vascular Control During Pregnancy : Extending Experimental Findings to Humans Circ. Res., September 1, 2000; 87(5): 344 - 345. [Full Text] [PDF]
|
|
|
|
|
|
S. H. Nelson, O. S. Steinsland, Y. Wang, C. Yallampalli, Y.-L. Dong, and J. M. Sanchez Increased Nitric Oxide Synthase Activity and Expression in the Human Uterine Artery During Pregnancy Circ. Res., September 1, 2000; 87(5): 406 - 411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Buhimschi, C. Yallampalli, C. S. Buhimschi, G. R. Saade, and R. E. Garfield Distinct regulation of nitric oxide and cyclic guanosine monophosphate production by steroid hormones in the rat uterus Mol. Hum. Reprod., May 1, 2000; 6(5): 404 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jovanovic, L. Grbovic, and A. Jovanovic Pregnancy is associated with altered response to neuropeptide Y in uterine artery Mol. Hum. Reprod., April 1, 2000; 6(4): 352 - 360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Komers, T. T. Oyama, J. G. Chapman, K. M. Allison, and S. Anderson Effects of Systemic Inhibition of Neuronal Nitric Oxide Synthase in Diabetic Rats Hypertension, February 1, 2000; 35(2): 655 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Itoh, J. Zheng, I. M. Bird, K. Nakao, and R. R. Magness Basic FGF decreases clearance receptor of natriuretic peptides in fetoplacental artery endothelium Am J Physiol Regulatory Integrative Comp Physiol, August 1, 1999; 277(2): R541 - R547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.L. Purcell, R. Given, K Chwalisz, and R.E. Garfield Nitric oxide synthase distribution during implantation in the mouse Mol. Hum. Reprod., May 1, 1999; 5(5): 467 - 475. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. White, R. E. McCullough, R. Dyckes, A. D. Robertson, and L. G. Moore Effects of pregnancy and chronic hypoxia on contractile responsiveness to alpha 1-adrenergic stimulation J Appl Physiol, December 1, 1998; 85(6): 2322 - 2329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Vagnoni, C. E. Shaw, T. M. Phernetton, B. M. Meglin, I. M. Bird, and R. R. Magness Endothelial vasodilator production by uterine and systemic arteries. III. Ovarian and estrogen effects on NO synthase Am J Physiol Heart Circ Physiol, November 1, 1998; 275(5): H1845 - H1856. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
