AJP - Heart and Circulatory Physiology, Vol 272, Issue 4 1725-H1729, Copyright © 1997 by American Physiological Society

ARTICLES

Cytokine-induced leukocyte rolling in mouse cremaster muscle arterioles in P-selectin dependent

H. Thorlacius, L. Lindbom and J. Raud
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.

Leukocyte rolling and adhesion are generally observed in venules but rarely observed in arterioles. With the use of intravital microscopy, we found that a 4-h treatment with interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) dose dependently induced leukocyte rolling and adhesion in arterioles of the mouse cremaster muscle. The rolling response lasted more than 24 h and was completely inhibited by treatment with the sulfated polysaccharide fucoidin. Moreover, we found that costimulation with IL-1beta and TNF-alpha for 4 h synergistically increased arteriolar leukocyte rolling, i.e., threshold doses of IL-1beta and TNF-alpha together caused a more than 10-fold increase of rolling in arterioles compared with the sum of the individual responses. This rolling interaction was abolished by treatment with a monoclonal antibody directed against P-selectin (RB40.34), but it apparently was unaffected by a monoclonal antibody against L-selectin (MEL-14). Taken together, our functional data show that IL-1beta and TNF-alpha separately induce and synergistically increase P-selectin-dependent leukocyte rolling and firm adhesion in mouse cremaster arterioles.

This article has been cited by other articles:

				R. Sumagin, E. Lomakina, and I. H. Sarelius Leukocyte-endothelial cell interactions are linked to vascular permeability via ICAM-1-mediated signaling Am J Physiol Heart Circ Physiol, September 1, 2008; 295(3): H969 - H977. [Abstract] [Full Text] [PDF]

				R. Sumagin and I. H. Sarelius A role for ICAM-1 in maintenance of leukocyte-endothelial cell rolling interactions in inflamed arterioles Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2786 - H2798. [Abstract] [Full Text] [PDF]

				A. L. Patrick, J. Rullo, S. Beaudin, P. Liaw, and A. E. Fox-Robichaud Hepatic leukocyte recruitment in response to time-limited expression of TNF-{alpha} and IL-1beta Am J Physiol Gastrointest Liver Physiol, October 1, 2007; 293(4): G663 - G672. [Abstract] [Full Text] [PDF]

				R. Sumagin and I. H. Sarelius TNF-{alpha} activation of arterioles and venules alters distribution and levels of ICAM-1 and affects leukocyte-endothelial cell interactions Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2116 - H2125. [Abstract] [Full Text] [PDF]

				D. H. Broide, G. Stachnick, D. Castaneda, J. Nayar, and P. Sriramarao Inhibition of Eosinophilic Inflammation in Allergen-Challenged TNF Receptor p55/p75- and TNF Receptor p55-Deficient Mice Am. J. Respir. Cell Mol. Biol., March 1, 2001; 24(3): 304 - 311. [Abstract] [Full Text] [PDF]

				S. C. Tromp, M. G. A. oude Egbrink, R. P. M. Dings, S. van Velzen, D. W. Slaaf, H. F. P. Hillen, G. J. Tangelder, R. S. Reneman, and A. W. Griffioen Tumor angiogenesis factors reduce leukocyte adhesion in vivo Int. Immunol., May 1, 2000; 12(5): 671 - 676. [Abstract] [Full Text] [PDF]

				D. H. Broide, K. Campbell, T. Gifford, and P. Sriramarao Inhibition of eosinophilic inflammation in allergen-challenged, IL-1 receptor type 1-deficient mice is associated with reduced eosinophil rolling and adhesion on vascular endothelium Blood, January 1, 2000; 95(1): 263 - 269. [Abstract] [Full Text] [PDF]

				A. S. DE VRIESE, K. ENDLICH, M. ELGER, N. H. LAMEIRE, R. C. ATKINS, H. Y. LAN, A. RUPIN, W. KRIZ, and M. W. STEINHAUSEN The Role of Selectins in Glomerular Leukocyte Recruitment in Rat Anti-Glomerular Basement Membrane Glomerulonephritis J. Am. Soc. Nephrol., December 1, 1999; 10(12): 2510 - 2517. [Abstract] [Full Text]