| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Heart and Circulatory Physiology, Vol 272, Issue 2 657-H668, Copyright © 1997 by American Physiological Society
ARTICLES |
H. Satoh, L. A. Blatter and D. M. Bers
Department of Physiology, Loyola University Chicago, Maywood, Illinois 60153, USA.
In heart, spontaneous local increases in cytosolic Ca2+ concentration ([Ca2+]i) called "Ca2+ sparks" may be fundamental events underlying both excitation-contraction coupling and resting Ca2+ leak from the sarcoplasmic reticulum (SR). In this study, resting Ca2+ sparks were analyzed in rabbit and rat ventricular myocytes with laser scanning confocal microscopy and the fluorescent Ca2+ indicator fluo 3. During the first 20 s of rest after regular electrical stimulation, both the frequency of Ca2+ sparks and SR Ca2+ content gradually decreased in rabbit. When rabbit SR Ca2+ content was decreased by reduction of stimulation rate. the initial resting spark frequency was also decreased, even though resting [Ca2+]i was unchanged. The rest-dependent decrease in spark frequency in rabbit cells was prevented by inhibition of Na+/Ca2+ exchange (which also prevents SR Ca2+ depletion during rest). These results suggest that elevation of SR Ca2+ content can increase Ca2+ spark frequency. In contrast to rabbit cells, 20 s of rest produced a gradual increase in spark frequency in rat cells, although SR Ca2+ content was constant and Ca2+ influx was completely prevented. This indicates that there is a time-dependent increase in spark probability during rest that is independent of [Ca2+]i or SR Ca2+. This effect was also apparent in rabbit cells when SR Ca2+ depletion was prevented by blocking Na+/Ca2+ exchange. Stimulation of Ca2+ extrusion via Na+/Ca2+ exchange in the rat (by Ca2+-free superfusion, which slowly depletes SR Ca2+ content) converted the normal rest-dependent increase in spark frequency to a decrease. The amplitude of individual Ca2+ sparks increased with increasing SR Ca2+ content. In the Ca2+-overloaded state, fusion of sparks or long-lasting localized increases of [Ca2+]i were observed with increased spark frequency. We conclude that the resting frequency of Ca2+ sparks can be independently affected by changes in SR Ca2+ content, [Ca2+]i, or rest period. The latter may reflect recovery of the SR Ca2+ release channels from inactivation or adaptation.
This article has been cited by other articles:
|
|
 |
|
  H. Cheng and W. J. Lederer Calcium Sparks Physiol Rev, October 1, 2008; 88(4): 1491 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Shutt and S. E. Howlett Hypothermia increases the gain of excitation-contraction coupling in guinea pig ventricular myocytes Am J Physiol Cell Physiol, September 1, 2008; 295(3): C692 - C700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Picht, A. V. Zima, L. A. Blatter, and D. M. Bers SparkMaster: automated calcium spark analysis with ImageJ Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1073 - C1081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-A. Bray, N. A. Geisse, and K. K. Parker Multidimensional Detection and Analysis of Ca2+ Sparks in Cardiac Myocytes Biophys. J., June 15, 2007; 92(12): 4433 - 4443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Copello, A. V. Zima, P. L. Diaz-Sylvester, M. Fill, and L. A. Blatter Ca2+ entry-independent effects of L-type Ca2+ channel modulators on Ca2+ sparks in ventricular myocytes Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2129 - C2140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Armoundas, J. Rose, R. Aggarwal, B. D. Stuyvers, B. O'Rourke, D. A. Kass, E. Marban, S. R. Shorofsky, G. F. Tomaselli, and C. William Balke Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1607 - H1618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Howlett, S. A. Grandy, and G. R. Ferrier Calcium spark properties in ventricular myocytes are altered in aged mice Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1566 - H1574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Ferrier, R. H. Smith, and S. E. Howlett Calcium sparks in mouse ventricular myocytes at physiological temperature Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1495 - H1505. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. V. Remillard, W.-M. Zhang, L. A. Shimoda, and J. S. K. Sham Physiological properties and functions of Ca2+ sparks in rat intrapulmonary arterial smooth muscle cells Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L433 - L444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Sobie, K. W. Dilly, J. dos Santos Cruz, W. J. Lederer, and M. S. Jafri Termination of Cardiac Ca2+ Sparks: An Investigative Mathematical Model of Calcium-Induced Calcium Release Biophys. J., July 1, 2002; 83(1): 59 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Bers Calcium and Cardiac Rhythms: Physiological and Pathophysiological Circ. Res., January 11, 2002; 90(1): 14 - 17. [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Ziolo, H. Katoh, and D. M. Bers Expression of Inducible Nitric Oxide Synthase Depresses {beta}-Adrenergic-Stimulated Calcium Release From the Sarcoplasmic Reticulum in Intact Ventricular Myocytes Circulation, December 11, 2001; 104(24): 2961 - 2966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Ziolo, H. Katoh, and D. M. Bers Positive and negative effects of nitric oxide on Ca2+ sparks: influence of beta -adrenergic stimulation Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2295 - H2303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sauer, T. Theben, J. Hescheler, M. Lindner, M. C. Brandt, and M. Wartenberg Characteristics of calcium sparks in cardiomyocytes derived from embryonic stem cells Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H411 - H421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Schlotthauer and D. M. Bers Sarcoplasmic Reticulum Ca2+ Release Causes Myocyte Depolarization : Underlying Mechanism and Threshold for Triggered Action Potentials Circ. Res., October 27, 2000; 87(9): 774 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Katoh, K. Schlotthauer, and D. M. Bers Transmission of Information From Cardiac Dihydropyridine Receptor to Ryanodine Receptor : Evidence From BayK 8644 Effects on Resting Ca2+ Sparks Circ. Res., July 21, 2000; 87(2): 106 - 111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Satoh, K. S. Ginsburg, K. Qing, H. Terada, H. Hayashi, and D. M. Bers KB-R7943 Block of Ca2+ Influx Via Na+/Ca2+ Exchange Does Not Alter Twitches or Glycoside Inotropy but Prevents Ca2+ Overload in Rat Ventricular Myocytes Circulation, March 28, 2000; 101(12): 1441 - 1446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Pogwizd, M. Qi, W. Yuan, A. M. Samarel, and D. M. Bers Upregulation of Na+/Ca2+ Exchanger Expression and Function in an Arrhythmogenic Rabbit Model of Heart Failure Circ. Res., November 26, 1999; 85(11): 1009 - 1019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Haddock, W. A. Coetzee, E. Cho, L. Porter, H. Katoh, D. M. Bers, M. S. Jafri, and M. Artman Subcellular [Ca2+]i Gradients During Excitation-Contraction Coupling in Newborn Rabbit Ventricular Myocytes Circ. Res., September 3, 1999; 85(5): 415 - 427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Blaustein and W. J. Lederer Sodium/Calcium Exchange: Its Physiological Implications Physiol Rev, July 1, 1999; 79(3): 763 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Carmeliet Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias Physiol Rev, July 1, 1999; 79(3): 917 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Halet, P. Viard, J.-L. Morel, J. Mironneau, and C. Mironneau Effects of hindlimb suspension on cytosolic Ca2+ and [3H]ryanodine binding in cardiac myocytes Am J Physiol Heart Circ Physiol, April 1, 1999; 276(4): H1131 - H1136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Shorofsky, R. Aggarwal, M. Corretti, J. M. Baffa, J. M. Strum, B. A. Al-Seikhan, Y. M. Kobayashi, L. R. Jones, W. G. Wier, and C. W. Balke Cellular Mechanisms of Altered Contractility in the Hypertrophied Heart : Big Hearts, Big Sparks Circ. Res., March 5, 1999; 84(4): 424 - 434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Layland and J. C. Kentish Positive force- and [Ca2+]i-frequency relationships in rat ventricular trabeculae at physiological frequencies Am J Physiol Heart Circ Physiol, January 1, 1999; 276(1): H9 - H18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Satoh, H. Katoh, P. Velez, M. Fill, and D. M. Bers Bay K 8644 Increases Resting Ca2+ Spark Frequency in Ferret Ventricular Myocytes Independent of Ca Influx : Contrast With Caffeine and Ryanodine Effects Circ. Res., December 14, 1998; 83(12): 1192 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huser, D. M. Bers, and L. A. Blatter Subcellular properties of [Ca2+]i transients in phospholamban-deficient mouse ventricular cells Am J Physiol Heart Circ Physiol, May 1, 1998; 274(5): H1800 - H1811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Shorofsky, L. Izu, W. G. Wier, and C. W. Balke Ca2+ Sparks Triggered by Patch Depolarization in Rat Heart Cells Circ. Res., March 9, 1998; 82(4): 424 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Mironneau, F. Coussin, L. H. Jeyakumar, S. Fleischer, C. Mironneau, and N. Macrez Contribution of Ryanodine Receptor Subtype 3 to Ca2+ Responses in Ca2+-overloaded Cultured Rat Portal Vein Myocytes J. Biol. Chem., March 30, 2001; 276(14): 11257 - 11264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Shannon, G. Chu, E. G. Kranias, and D. M. Bers Phospholamban Decreases the Energetic Efficiency of the Sarcoplasmic Reticulum Ca Pump J. Biol. Chem., March 2, 2001; 276(10): 7195 - 7201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, E. G. Kranias, G. A. Mignery, and D. M. Bers Protein Kinase A Phosphorylation of the Ryanodine Receptor Does Not Affect Calcium Sparks in Mouse Ventricular Myocytes Circ. Res., February 22, 2002; 90(3): 309 - 316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Pogwizd, K. Schlotthauer, L. Li, W. Yuan, and D. M. Bers Arrhythmogenesis and Contractile Dysfunction in Heart Failure : Roles of Sodium-Calcium Exchange, Inward Rectifier Potassium Current, and Residual {beta}-Adrenergic Responsiveness Circ. Res., June 8, 2001; 88(11): 1159 - 1167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jane Lalli, J. Yong, V. Prasad, K. Hashimoto, D. Plank, G. J. Babu, D. Kirkpatrick, R. A. Walsh, M. Sussman, A. Yatani, et al. Sarcoplasmic Reticulum Ca2+ ATPase (SERCA) 1a Structurally Substitutes for SERCA2a in the Cardiac Sarcoplasmic Reticulum and Increases Cardiac Ca2+ Handling Capacity Circ. Res., July 20, 2001; 89(2): 160 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yang and D. S. Steele Effects of Cytosolic ATP on Ca2+ Sparks and SR Ca2+ Content in Permeabilized Cardiac Myocytes Circ. Res., September 14, 2001; 89(6): 526 - 533. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
