| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Heart and Circulatory Physiology, Vol 271, Issue 2 562-H570, Copyright © 1996 by American Physiological Society
ARTICLES |
V. M. Reddy, J. Wong, J. R. Liddicoat, M. Johengen, R. Chang and J. R. Fineman
Department of Cardiothoracic Surgery, University of California, San Francisco 94143-0106, USA.
To investigate early endothelial function associated with increased pulmonary blood flow, vascular shunts were placed between the ascending aorta and main pulmonary artery in 18 late-gestation fetal sheep. Four weeks after delivery, the lambs were instrumented to measure vascular pressures and blood flows, and blood was collected to measure plasma concentrations of guanosine 3',5'-cyclic monophosphate [cGMP, the second messenger to nitric oxide (NO)-mediated vasodilation] and L-arginine (the precursor for NO synthesis). The responses to the endothelium-dependent vasodilators acetylcholine (ACh, 1.0 microgram/kg) and ATP (0.1 mg.kg-1.min-1), the endothelium-independent vasodilators M & B-22948 (a cGMP-specific phosphodiesterase inhibitor, 2.5 mg/kg) and inhaled NO (40 ppm), and N omega-nitro-L-arginine (an inhibitor of NO synthase, 5 mg/kg) were then compared with responses in 12 age-matched controls. Vasodilator responses in control lambs were determined during pulmonary hypertension induced by U-46619 (a thromboxane A2 mimic). Shunted lambs displayed a selective impairment of endothelium-dependent pulmonary vasodilation, an augmented pulmonary vasoconstricting response to NO synthase inhibition, increased plasma cGMP concentrations, and decreased L-arginine concentrations. Taken together, these data suggest that lambs with pulmonary hypertension and increased pulmonary blood flow have early aberrations in endothelial function, as manifested by increased basal NO activity, that cannot be further increased by agonist-induced endothelium-dependent vasodilators.
This article has been cited by other articles:
|
|
 |
|
  P. E. Oishi, D. A. Wiseman, S. Sharma, S. Kumar, Y. Hou, S. A. Datar, A. Azakie, M. J. Johengen, C. Harmon, S. Fratz, et al. Progressive dysfunction of nitric oxide synthase in a lamb model of chronically increased pulmonary blood flow: a role for oxidative stress Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L756 - L766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sud, S. Sharma, D. A. Wiseman, C. Harmon, S. Kumar, R. C. Venema, J. R. Fineman, and S. M. Black Nitric oxide and superoxide generation from endothelial NOS: modulation by HSP90 Am J Physiol Lung Cell Mol Physiol, December 1, 2007; 293(6): L1444 - L1453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lakshminrusimha, D. Wiseman, S. M. Black, J. A. Russell, S. F. Gugino, P. Oishi, R. H. Steinhorn, and J. R. Fineman The role of nitric oxide synthase-derived reactive oxygen species in the altered relaxation of pulmonary arteries from lambs with increased pulmonary blood flow Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1491 - H1497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Grobe, S. M. Wells, E. Benavidez, P. Oishi, A. Azakie, J. R. Fineman, and S. M. Black Increased oxidative stress in lambs with increased pulmonary blood flow and pulmonary hypertension: role of NADPH oxidase and endothelial NO synthase Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1069 - L1077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Oishi, A. Azakie, C. Harmon, R. K. Fitzgerald, A. Grobe, J. Xu, K. Hendricks-Munoz, S. M. Black, and J. R. Fineman Nitric oxide-endothelin-1 interactions after surgically induced acute increases in pulmonary blood flow in intact lambs Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H1922 - H1932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Ross, P. Oishi, A. Azakie, S. Fratz, R. K. Fitzgerald, M. J. Johengen, C. Harmon, K. Hendricks-Munoz, J. Xu, S. M. Black, et al. Endothelial alterations during inhaled NO in lambs with pulmonary hypertension: implications for rebound hypertension Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L27 - L35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ovadia, O. Reinhartz, R. Fitzgerald, J. M. Bekker, M. J. Johengen, A. Azakie, S. Thelitz, S. M. Black, and J. R. Fineman Alterations in ET-1, not nitric oxide, in 1-week-old lambs with increased pulmonary blood flow Am J Physiol Heart Circ Physiol, February 1, 2003; 284(2): H480 - H490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N. Cornfield, E. R. Resnik, J. M. Herron, O. Reinhartz, and J. R. Fineman Pulmonary vascular K+ channel expression and vasoreactivity in a model of congenital heart disease Am J Physiol Lung Cell Mol Physiol, December 1, 2002; 283(6): L1210 - L1219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Black, L. S. Sanchez, E. Mata-Greenwood, J. M. Bekker, R. H. Steinhorn, and J. R. Fineman sGC and PDE5 are elevated in lambs with increased pulmonary blood flow and pulmonary hypertension Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1051 - L1057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Fike, M. R. Kaplowitz, and M. Bousamra II eNOS and prostanoid enzymes in lungs of newborn piglets with chronic aortopulmonary shunts Am J Physiol Lung Cell Mol Physiol, August 1, 2001; 281(2): L475 - L482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. F. BERGER, R. GEIGER, J. HESS, A. J. J. C. BOGERS, and W. J. MOOI Altered Arterial Expression Patterns of Inducible and Endothelial Nitric Oxide Synthase in Pulmonary Plexogenic Arteriopathy Caused by Congenital Heart Disease Am. J. Respir. Crit. Care Med., May 1, 2001; 163(6): 1493 - 1499. [Abstract] [Full Text]
|
|
|
|
|
|
R. H. Steinhorn, J. A. Russell, S. Lakshminrusimha, S. F. Gugino, S. M. Black, and J. R. Fineman Altered endothelium-dependent relaxations in lambs with high pulmonary blood flow and pulmonary hypertension Am J Physiol Heart Circ Physiol, January 1, 2001; 280(1): H311 - H317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Bennett, M. W. Eldridge, D. Zaghi, S. E. Zaghi, J. M. Milstein, and B. W. Goetzman Form and function of fetal and neonatal pulmonary arterial bifurcations Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H3047 - H3057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Gordon, T. R. Halla, C. D. Fike, and J. A. Madden Mediators of alkalosis-induced relaxation in pulmonary arteries from normoxic and chronically hypoxic piglets Am J Physiol Lung Cell Mol Physiol, January 1, 1999; 276(1): L155 - L163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Takaya, M Teraguchi, S Nogi, Y Ikemoto, and Y Kobayashi Relation between plasma nitrate and mean pulmonary arterial pressure in ventricular septal defect Arch. Dis. Child., December 1, 1998; 79(6): 498 - 501. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Black, J. R. Fineman, R. H. Steinhorn, J. Bristow, and S. J. Soifer Increased endothelial NOS in lambs with increased pulmonary blood flow and pulmonary hypertension Am J Physiol Heart Circ Physiol, November 1, 1998; 275(5): H1643 - H1651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. T. KANEKO, A. C. ARROLIGA, R. A. DWEIK, S. A. COMHAIR, D. LASKOWSKI, R. OPPEDISANO, M. J. THOMASSEN, and S. C. ERZURUM Biochemical Reaction Products of Nitric Oxide as Quantitative Markers of Primary Pulmonary Hypertension Am. J. Respir. Crit. Care Med., September 1, 1998; 158(3): 917 - 923. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
