AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 256: H539-H551, 1989;
0363-6135/89 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roos, K. P.
Right arrow Articles by Brady, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roos, K. P.
Right arrow Articles by Brady, A. J.

AJP - Heart and Circulatory Physiology, Vol 256, Issue 2 539-H551, Copyright © 1989 by American Physiological Society

ARTICLES

Stiffness and shortening changes in myofilament-extracted rat cardiac myocytes

K. P. Roos and A. J. Brady
Department of Physiology, School of Medicine, University of California, Los Angeles 90024-1760.

Sarcomere lengths, cell widths, volumes, stiffness, and regional striation uniformity were determined from isolated adult cardiac myocytes. Single cells were examined in the control saline solution followed by a sequence of relaxing, membrane skinning, and myofilament extraction solutions. Cell size and shape parameters were determined from freely dispersed myocytes, whereas stiffness was measured from myocytes attached to a perturbator and tension transducer with micropipettes. There were small changes in cell appearance, size, shape, and stiffness in the relaxing and skinning solutions. However, in 0.17-0.56 M KCl myosin extraction media, cell length declined significantly to 1.19 microns, and stiffness fell to 5-10% of control. The rate of cell shortening and stiffness decline was dependent on KCl concentration and pH. Subsequent exposure to higher ionic strength 0.60 M KI thin filament extraction media elicited additional decreases in stiffness (less than 5% of control) and cell length (0.98 micron). Cell shortening and stiffness decline have similar time courses under the same conditions, and they appear to coincide with A-band disassembly as indicated by electron micrographs. These data suggest that cardiac myocyte stiffness, size, and shape are determined in part by a stressed cytoskeleton that is associated with the myofilament apparatus.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. R. Zile, M. K. Cowles, J. M. Buckley, K. Richardson, B. A. Cowles, C. F. Baicu, G. Cooper IV, and V. Gharpuray
Gel stretch method: a new method to measure constitutive properties of cardiac muscle cells
Am J Physiol Heart Circ Physiol, June 1, 1998; 274(6): H2188 - H2202.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. L. Rigby, P. A. Hofmann, J. Zhong, H. R. Adams, and L. J. Rubin
Endotoxemia-induced myocardial dysfunction is not associated with changes in myofilament Ca2+ responsiveness
Am J Physiol Heart Circ Physiol, February 1, 1998; 274(2): H580 - H590.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
D. Fan, T. Wannenburg, and P. P. de Tombe
Decreased Myocyte Tension Development and Calcium Responsiveness in Rat Right Ventricular Pressure Overload
Circulation, May 6, 1997; 95(9): 2312 - 2317.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
K. Trombitas, J.-P. Jin, and H. Granzier
The Mechanically Active Domain of Titin in Cardiac Muscle
Circ. Res., October 1, 1995; 77(4): 856 - 861.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online