AJP - Heart and Circulatory Physiology, Vol 256, Issue 1 311-H314, Copyright © 1989 by American Physiological Society
Subtype switching of ANP receptors during in vitro culture of vascular cells
K. Uchida, T. Mizuno, M. Shimonaka, N. Sugiura, H. Hagiwara and S. Hirose
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Vascular receptors for atrial natriuretic peptide (ANP) have been
characterized immunochemically and found to be classified into two subtypes
that show differential localization and expression. An antiserum,
recognizing the protein core of the receptor, was raised against the
purified bovine lung ANP receptor and used for the immunochemical
subtyping. ANP receptors solubilized from the bovine jugular vein reacted
strongly with the antiserum as well as the homologous lung receptor;
however, the receptors from the aorta and carotid artery were recognized
only weakly, indicating the presence of two types of ANP receptor in the
vascular system. The one that reacts strongly with the antiserum and is
distributed mainly in the venous side is termed type I and the other
reacting weakly and predominating in the arteries, type II. The two
subtypes were also distinguishable in their ligand specificities; the type
I receptor showed a remarkably higher affinity for the ANP analogue
atriopeptin I than the type II receptor. Surprisingly, similar
immunochemical and biochemical analysis of the receptors on cultured
vascular smooth muscle and endothelial cells revealed that the arterial
cells originally expressing the type II receptor begin to express the type
I receptor when cultured in vitro.
