AJP - Heart and Circulatory Physiology, Vol 253, Issue 6 1462-H1469, Copyright © 1987 by American Physiological Society
Increased skin lymph protein clearance after a 6-h arterial bradykinin infusion
R. J. Mullins and R. W. Hudgens
Department of Surgery, University of Louisville Medical School, Kentucky.
When bradykinin (0.15-0.28 micrograms.kg-1.min-1) was infused into both
femoral arteries of 11 anesthetized dogs, skin lymph flows increased by
25-371% within 2 h, and mean lymph protein concentrations increased by
one-third. To determine whether, in addition to the initial increase in
permeability, a 6.5- to 10-h bradykinin infusion caused a sustained effect,
the bradykinin infusion into one hindpaw was stopped after 2 h (2HR),
whereas the contralateral hindpaw was infused continuously (CONT). Two
hours after the bradykinin infusion was stopped, Ringer lactate equal to
10% of the dog's body weight was given intravenously to further increase
lymph flow. After Ringer lactate infusion, increase in lymph protein
clearance from the CONT hindpaws was greater than that from the 2HR
hindpaws (change in clearance from before Ringer lactate infusion to final:
2HR, 6.9 +/- 1.4 to 8.8 +/- 1.1; CONT, 23.4 +/- 2.5 to 40.2 +/- 4.8
microliters/min). In the final lymph samples of the CONT, but not 2HR,
hindpaws, the lymph-to-plasma ratio for immunoglobulin G and immunoglobulin
M divided by the albumin lymph-to-plasma ratio exceeded the value of these
ratios in the base-line samples. An intravenous bolus of Evans blue dye was
given less than 2 h before the end of the experiment. The concentrations of
dye in the final lymph samples were greater in CONT hindpaws (12.6 +/- 3.7%
plasma equivalents) than in the 2HR hindpaws (1.1 +/- 0.5%). A continuous
6.5- to 10-h intra-arterial bradykinin infusion produced a sustained
increase of transvascular protein clearance in skin that is consistent with
a sustained increase in microvascular membrane permeability.
