AJP - Heart and Circulatory Physiology, Vol 248, Issue 1 26-H32, Copyright © 1985 by American Physiological Society
Peritoneal absorption of macromolecules studied by quantitative autoradiography
M. F. Flessner, J. D. Fenstermacher, R. G. Blasberg and R. L. Dedrick
Transport experiments of 125I-human serum albumin from the peritoneal
cavity to the plasma were conducted in 200-g female rats. Blood and
peritoneal samples were collected at intervals over 2-3 h. After death and
rapid freezing of the animal, transverse sections were cut in a
cryomicrotome from several tissues surrounding the peritoneal cavity, and
the distribution of the labeled albumin was measured by computerized
quantitative macroautoradiography. Tissue concentrations (counts/min per
wet tissue wt) in parietal tissues (anterior abdominal wall and the
diaphragm) were relatively constant versus distance from the peritoneum and
represented a large fraction (0.5-1.0) of the concentration in the
peritoneal cavity. Fractional concentrations in visceral tissues (liver,
stomach, intestine) decreased from 0.20-0.35 at the peritoneal surface to
0.03-0.06 at a distance of 900 micron from the peritoneum. Uterine tissue
concentrations lay between those of the parietal tissues and those of the
viscera. The data are related to mechanisms of interstitial and lymphatic
transport in these tissues.
