AJP - Heart Myographs and Tissue organ baths
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Am J Physiol Heart Circ Physiol 247: H1018-H1026, 1984;
0363-6135/84 $5.00
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AJP - Heart and Circulatory Physiology, Vol 247, Issue 6 1018-H1026, Copyright © 1984 by American Physiological Society

ARTICLES

A new method for preparation of isolated single adult myocytes

G. Bkaily, N. Sperelakis and J. Doane

A new technique for isolation of single cardiac cells from adult rat ventricles is described. The mechanical method is modified from the method of Jacobson (Cell Struct. Funct. 2: 1-9, 1977) by employing an enzyme-free dispersion medium containing Sr2+ or Ba2+ to replace Ca2+. This method produces a high yield of isolated rod-shaped adult mammalian cardiomyocytes that do not contract spontaneously, have a normal resting potential, and are Ca2+ tolerant. This method represents a promising technique for isolation of single, viable, intact, adult myocytes.


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 Am. J. Physiol. Heart Circ. Physiol.Home page
M. G. Garcia-Webb, A. J. Taberner, N. C. Hogan, and I. W. Hunter
A modular instrument for exploring the mechanics of cardiac myocytes
Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H866 - H874.
[Abstract] [Full Text] [PDF]


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